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CD8a-EGFP

Plasmid

#86051

Purpose

A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-UbxD8

Plasmid

#53777

Purpose

express FLAG-tagged human UbxD8 gene

Depositor

Yihong Ye

Article

Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

Insert

UbxD8 (FAF2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only
AIRAP 9: PSMD2.GST.V2.pcDNA3

Plasmid

#21799

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD2 (PSMD2 Human)

Tags

GST

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9wt

Plasmid

#20368

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pBR322-Pdp1_NTD-Cas9

Plasmid

#198275

Purpose

For loading SpCas9 into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cas9

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NlucP

Plasmid

#162595

Purpose

C-terminally PEST-tagged NanoLuc under TRE3G promoter

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

NanoLuc-PEST

Tags

PEST

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLCAG EGFP

Plasmid

#14857

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2002 Feb 19. 9

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only
OCT4-2A-mOrange

Plasmid

#66986

Purpose

Generate OCT4 mOrange reporter line without selection cassette

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

2A-mOrange

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-PRAS40

Plasmid

#15481

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GR100

Plasmid

#84908

Purpose

induce expression of dipeptide repeat GR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GR100 (C9orf72 Synthetic)

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-PCAF

Plasmid

#63705

Purpose

retroviral expression of human PCAF in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

PCAF (KAT2B Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

E452G

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pQP-iRIS

Plasmid

#102584

Purpose

Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Backbone(HD)

Plasmid

#27964

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(HD)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-GFP-HiBiT

Plasmid

#198317

Purpose

Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-GFP-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec24D

Plasmid

#32678

Depositor

Henry Lester

Article

Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

Insert

Sec24D (SEC24D Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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pRL K-ras 3'UTR

Plasmid

#14804

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

K-ras 3'UTR (Kras Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag FKHR H215R

Plasmid

#13509

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR H215R (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H215R

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only
CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

CFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pISce1-D2EGFP

Plasmid

#32674

Depositor

Michael Tsang

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

EGFP

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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