We narrowed to 9,400 results for: Coli

1. OmpT

   Plasmid

   #213137

   Purpose

   OmpT_native membrane expression

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   OmpT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMVS1111A:PhmtB-bgaB

   Plasmid

   #205998

   Purpose

   Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

   Depositor

   Bastian Molitor

   Article

   Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

   Insert

   thermostable beta-galactosidase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PhmtB

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. VV001: WT Arch-3 in pET28b

   Plasmid

   #58487

   Purpose

   Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

   Depositor

   Adam Cohen

   Article

   Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Archaerhodopsin-3 (with C-terminal His tag)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRBBm59

   Plasmid

   #48115

   Purpose

   Shuttle vector E. coli/B.meg.

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

   Insert

   promoter sacB - gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-trAtCCD8(pYL735)

   Plasmid

   #178286

   Purpose

   Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. VV020: WT Arch-3 - eGFP in pET28b

   Plasmid

   #58488

   Purpose

   Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

   Depositor

   Adam Cohen

   Article

   Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Archaerhodopsin-3
   

   Use

   Tags

   eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Atg24 1B

   Plasmid

   #197046

   Purpose

   His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

   Insert

   Atg24
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. BbZIP-cALFA

   Plasmid

   #160498

   Purpose

   For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   BbZIP
   

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. His6-Twin-Strep-SUMO-pET28a-EfaCas2

   Plasmid

   #112794

   Purpose

   To express enterococcus faecalis Cas2 protein in E. coli

   Depositor

   Ailong Ke

   Article

   Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

   Insert

   EfaCas2
   

   Use

   Tags

   His6-Twin-Strep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB1C3-H3

    Plasmid

    #109381

    Purpose

    Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPBO.ACT003

    Plasmid

    #182713

    Purpose

    aTc inducible dCasRx-IF3

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 3

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHL 246

    Plasmid

    #53014

    Purpose

    AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    fimS (off orientation)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. PET28a-cpLumiLuc-Rapamycin sensor

    Plasmid

    #186292

    Purpose

    Expression Rapamycin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-Rapamycin sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF-1b-S14

    Plasmid

    #128596

    Purpose

    Expresses tagless 30S ribosomal subunit protein S14 in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S14 (rpsN E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD/HisB-PhoCl

    Plasmid

    #87688

    Purpose

    Express PhoCl in E. coli

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPM2212

    Plasmid

    #167552

    Purpose

    Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

    Depositor

    Renato Morona

    Article

    Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

    Insert

    rfb region of Shigella flexneri serotype 2a
    

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHL 1903

    Plasmid

    #53020

    Purpose

    AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    AAV, Asp terminator, and fimS

    Expression

    Bacterial

    Mutation

    Promoter

    fimS

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pHis-zfCSF3a

    Plasmid

    #65612

    Purpose

    express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

    Insert

    Csf3a (csf3a Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pSEVA221MBPc-AID-T7RNAP

    Plasmid

    #167975

    Purpose

    Expresses fusion protein MBPc-AID-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    MBPc-AID-T7RNAP
    

    Use

    Tags

    MBPc and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBAD-TRP120

    Plasmid

    #71732

    Purpose

    inducible expression of TRP120 in E. coli

    Depositor

    Jere McBride

    Article

    Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

    Insert

    TRP120
    

    Use

    Tags

    6xHIS and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Use

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-6xHis-GFP(+24)

    Plasmid

    #199163

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+24)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXD70Tet(DadR)-DadR

    Plasmid

    #191636

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET47b-T7-dAsCas12f

    Plasmid

    #204641

    Purpose

    Expression of an inactive AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    inactive AsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D225A

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a SV40 ST full length

    Plasmid

    #132636

    Purpose

    expresses SV40 ST full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

    Insert

    SV40 small t antigen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK240

    Plasmid

    #71127

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    GyrA intein and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDB328

    Plasmid

    #113672

    Purpose

    expresses Ulp1_R4 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R4
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRBBm69

    Plasmid

    #48116

    Purpose

    Shuttle vector E. coli/B.meg.

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    promoter sacB - sacB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LoriT

    Plasmid

    #163691

    Purpose

    Modified L4440 containing now CamR and oriT for conjugation

    Depositor

    Baris Tursun

    Article

    Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

    Insert

    oriT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge