We narrowed to 5,030 results for: codon optimized

1. pBS-Hsp70-Cas9

   Plasmid

   #46294

   Purpose

   A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

   Depositor

   Jill Wildonger, Melissa Harrison, Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x Flag

   Expression

   Insect

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Hsp70

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

   Plasmid

   #139987

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSW002-Pc-mNeonGreen

   Plasmid

   #205016

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

   Plasmid

   #134341

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIC3
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

   Plasmid

   #133801

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA4
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

   Plasmid

   #133802

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA5
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

   Plasmid

   #134337

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA16_Efa
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

   Plasmid

   #134332

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA16_Lmo
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

   Plasmid

   #134340

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIC1
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB1679 CMV-HIVNES-GS-Cas13bt3

    Plasmid

    #176317

    Purpose

    CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt3
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSN067

    Plasmid

    #101748

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKII-GAL4-iLight-VP16

    Plasmid

    #170274

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Comments

    Promoter

    CaMKII

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSN068

    Plasmid

    #101749

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSW003-PpsbA-mNeonGreen

    Plasmid

    #205019

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    PpsbA

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

    Plasmid

    #134333

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA17_Efa
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a-ncOGT-TPR

    Plasmid

    #190822

    Purpose

    For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    C467X. Also codon optimized for expression in E.c…

    Promoter

    T7

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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