We narrowed to 1,583 results for: pbluescript

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-2033/+750 hTSP1 promoter CAT

Plasmid

#15815

Depositor

Paul Bornstein

Insert

TSP1 promoter (THBS1 Human)

Expression

Mammalian

Available Since

Oct. 3, 2007

Availability

Academic Institutions and Nonprofits only
pBS mouse c-Myc

Plasmid

#14972

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (Myc Mouse)

Expression

Bacterial

Mutation

Nucleotides 2578-3294

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pBS hTSP-4

Plasmid

#13001

Depositor

Jack Lawler

Article

Lawler et al J Biol Chem. 1995 Feb 10. 270(6):2809-14

Insert

TSP-4 (THBS4 Human)

Expression

Bacterial

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_iPE-N

Plasmid

#214734

Purpose

encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

Depositor

Gil Westmeyer

Article

Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

Insert

CAG_iPE-N_bGH

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMS12: pDonor(AvCAST)

Plasmid

#168145

Purpose

AvCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST right end (RE)
AvCAST left end (LE)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP PR-D25A

Plasmid

#101334

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Mutation

Protease D25A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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aP2-GFPtpz

Plasmid

#114211

Purpose

aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage

Depositor

David Rowe

Article

Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101

Insert

aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Synthetic, Aequorea victoria, Mouse)

Tags

None

Expression

Mammalian

Promoter

aP2/Fabp4

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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HyGCaMP6s

Plasmid

#102558

Purpose

Expression of GCaMP6s in Hydra

Depositor

Rafael Yuste

Article

Dupre et al Curr Biol. 2017 Apr 24;27(8):1085-1097.

Insert

GCaMP6s

Use

Hydra expression

Tags

Actin/GCaMP6s fusion protein (C terminal on backb…

Available Since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRosa26-Acceptor (AG103)

Plasmid

#99632

Purpose

To insert the Tre-Tight or UAS ifgMosaic Donor vectors

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OK+9MS

Plasmid

#80482

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [1-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSKB-

Plasmid

#62540

Purpose

Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Bacterial

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pC-PTP-NEO

Plasmid

#51710

Purpose

contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Available Since

March 12, 2014

Availability

Academic Institutions and Nonprofits only

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pBlue-TAL

Plasmid

#49401

Purpose

The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

Depositor

Michal Zurovec

Article

Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

Type

Empty backbone

Use

TALEN

Expression

Insect

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pFT1.2

Plasmid

#46140

Depositor

Wenbiao Chen

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

mCherry

Use

Cre/Lox; Mutagenic gene trap for zebrafish

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pHyVec11

Plasmid

#34794

Depositor

Rob Steele

Type

Empty backbone

Use

Hydra expression

Tags

Intergenic region between Hydra replication facto…

Promoter

Hydra vulgaris actin promoter

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pmSfrp2

Plasmid

#31249

Depositor

Jeremy Nathans

Article

Rattner et al Proc Natl Acad Sci U S A. 1997 Apr 1. 94

Insert

Sfrp2 (Sfrp2 Mouse)

Use

Cdna for in situ hybridization

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pDRAV-1

Plasmid

#26746

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Type

Empty backbone

Use

Cre/Lox; Flp/frt; phic31/attb-attp

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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