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pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PE7-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-EGFP

Plasmid

#80945

Purpose

human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Expression

Mammalian

Promoter

CAG promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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TRE-mCherry

Plasmid

#92202

Purpose

Fluorescent protein reporter construct

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMVTNT PINK1 N-myc

Plasmid

#13313

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

myc

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Publication

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Tags

His

Expression

Bacterial

Promoter

T5/Lac

Available since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hDlx-GqDREADD-dTomato-Fishell-4

Plasmid

#83897

Purpose

Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV1, AAV9, and AAV Retrograde

Insert

hM3Dq

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pnos-Cas9-nos

Plasmid

#62208

Purpose

Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

nanos

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

Plasmid

#188766

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJR104

Plasmid

#187243

Purpose

Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

Use

Lentiviral

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSGAb-km

Plasmid

#121999

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Publication

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HP17_KO7

Plasmid

#120346

Purpose

Helper plasmid for production of phagemid ssDNA, based on M13KO7

Depositor

Hendrik Dietz

Publication

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13KO7 helper phage genome

Expression

Bacterial

Promoter

native phage promoters

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV-CMV-SOD2-2A-Catalase-WPRE

Plasmid

#67635

Purpose

AAV vector expressing both SOD2 and mitochondrial targeted Catalase

Depositor

Connie Cepko

Publication

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

SOD-2A-Catalase (Sod2, Cat Mouse)

Use

AAV

Mutation

Deleted the Peroxisome Targeting Signal from Cata…

Promoter

CMV

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Publication

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hsyn-GRAB_5-HT1.0

Plasmid

#140552

Purpose

Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

Depositor

Yulong Li

Publication

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Has service

AAV9

Insert

5-HT sensor GRAB_5-HT1.0

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-15F11-HA-Halo

Plasmid

#129592

Purpose

Encodes anti-HA scFv (anti-HA frankenbody) fused with HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDD284

Plasmid

#66825

Purpose

TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

TagRFP-T-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized TagRFP-T

Expression

Worm

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles