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Showing: 6271 - 6300 of 32932 results

  3. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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  6. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Expression
    Mammalian
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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  7. dCas12Max-ABE

    Plasmid
    #195335
    Purpose
    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-ABE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195335-plasmid-map-sequence-id-383124

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  8. gGBEv6.3-SpCas9

    Plasmid
    #202629
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpCas9(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…
    Promoter
    hU6, EF1a, CBh
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202629-plasmid-map-sequence-id-398425

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  9. pEf1a-superDn29-dCas9-P2A-GFP

    Plasmid
    #247156
    Purpose
    Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    superDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247156-plasmid-map-sequence-id-491689

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  11. pvPE-V4

    Plasmid
    #240288
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240288-plasmid-map-sequence-id-488805

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  12. gTBEv3

    Plasmid
    #220619
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220619-plasmid-map-sequence-id-436981

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  13. gGBEv6.3-SpG

    Plasmid
    #202630
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpG(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    hU6, EF1a, CBh
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202630-plasmid-map-sequence-id-398429

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  14. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  15. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

    Plasmid
    #246642
    Purpose
    EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.
    Depositor
    Koen Breyne
    Article
    Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70
    Insert
    cleavable E-NoMi-Red
    Use
    Lentiviral
    Tags
    FLAG-Nanoluciferase and mCherry
    Expression
    Mammalian
    Promoter
    EF-1alpha
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246642-plasmid-map-sequence-id-489517

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  18. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  23. TRE3G-PE2-P2A-BFP

    Plasmid
    #231580
    Purpose
    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein
    Depositor
    Jesse Engreitz
    Article
    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.
    Insert
    PE2-P2A-BFP
    Use
    Lentiviral
    Tags
    P2A-BFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231580-plasmid-map-sequence-id-462114

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  25. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  26. pHRIG-Akt1

    Plasmid
    #53583
    Purpose
    lentiviral expression of constitutively active human Akt1 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    cAkt1 (AKT1 Human)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available Since
    Oct. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53583-plasmid-map-sequence-id-448192

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  28. FKBP*-PTEN

    Plasmid
    #86781
    Purpose
    Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
    Depositor
    Britta Eickholt
    Article
    An et al PLoS One. 2015 Dec 30;10(12):e0145783. d
    Insert
    PTEN (PTEN Human)
    Tags
    FKBP* (F36V and L106P) and linker
    Expression
    Mammalian
    Available Since
    Feb. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86781-plasmid-map-sequence-id-169199

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  30. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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Showing: 6271 - 6300 of 32932 results