We narrowed to 6,963 results for: RAP

1. im:7163548_R (OZ572)

   Plasmid

   #35212

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting im:7163548 (im:7163548 Zebrafish)
   

   Use

   Zebrafish targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pT3-EF1A-MYC-IRES-luc

   Plasmid

   #129775

   Purpose

   Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

   Depositor

   Amaia Lujambio

   Article

   Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

   Insert

   human MYC (MYC Human)
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS-CoV-2 S HexaPro

   Plasmid

   #154754

   Purpose

   Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

   Depositor

   Jason McLellan

   Article

   Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

   Insert

   Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pLY095-EFS-ERBB2 HER2-Blast-WPRE

   Plasmid

   #192196

   Purpose

   Lenti-HER2-OE-Blast

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-HER2-OE-Blast (ERBB2 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

   Plasmid

   #229770

   Purpose

   Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
   

   Use

   Luciferase

   Tags

   LgBiT

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

   Plasmid

   #229769

   Purpose

   Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
   

   Use

   Luciferase

   Tags

   SmBiT

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5)

   Plasmid

   #229768

   Purpose

   Human Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
   

   Use

   Luciferase

   Tags

   LgBiT

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

   Plasmid

   #229767

   Purpose

   Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
   

   Use

   Luciferase

   Tags

   SmBiT

   Expression

   Mutation

   See Depositor Comments

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Sport6-CD63-pHluorin

   Plasmid

   #130901

   Purpose

   Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHluorin (CD63 Human, Aequorea victoria)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   pHluorin inserted between Gln-36 and Leu-37 in ex…

   Promoter

   CMV

   Available since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX401-INK4A

    Plasmid

    #121919

    Purpose

    Doxycycline-inducible expression of p16-INK4A product of CDKN2A

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    p16-INK4A (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tet ON

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid

    #124536

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Inserts

    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUW-VenusFlag-hCD44

    Plasmid

    #211824

    Purpose

    Express CD44 in mammalian cells

    Depositor

    Haian Fu

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    N-Terminal Venus and Flag tags

    Expression

    Mammalian

    Mutation

    isoform corresponds to UniProt ID P16070-1 with a…

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 Human, aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pINDUCER20 MEF2C

    Plasmid

    #89717

    Purpose

    Lentiviral gene expression vector for doxycycline inducible MEF2C expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Sport6-CD81-pHuji

    Plasmid

    #130904

    Purpose

    Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHuji (CD81 Human, Synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Leu-49 and Gly-50 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

    Plasmid

    #192190

    Purpose

    HER2 CAR AAV vector PRODH2 KI (pLY080b)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
    

    Use

    AAV; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid

    #132389

    Purpose

    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY57_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2-TRAC-RHA(3166mut)

    Plasmid

    #192188

    Purpose

    CD22 CAR AAV vector PRODH2 KI (pLY057)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    CD22 CAR AAV vector PRODH2 KI (pLY057) (CD22 Synthetic)
    

    Use

    AAV; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid

    #132393

    Purpose

    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Proline 301 to Leucine (P301L)

    Promoter

    TREtight

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLY59_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2(stop)-TRAC-RHA(3166mut)

    Plasmid

    #192189

    Purpose

    CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059) (CD22 Synthetic)
    

    Use

    AAV; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid

    #171802

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCag SE (Self-excising) FlpO-2A-Cre EV

    Plasmid

    #130986

    Purpose

    episomal expression of FlpO and Cre recombinases, self excising

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    FlpO-2A-Cre
    

    Use

    Cre/Lox and Unspecified; Episomal expression vect…

    Tags

    Expression

    Mammalian

    Mutation

    protamine intron added to FlpO to prevent bacteri…

    Promoter

    CMV/Chick β-actin (CAG)

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3_N-DYK_MTHFD1-NLS

    Plasmid

    #133023

    Purpose

    NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

    Depositor

    Stefan Kubicek

    Article

    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE

    Plasmid

    #192202

    Purpose

    Retro-HER2CAR-PRODH2

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-HER2CAR-PRODH2 (ERBB2 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-IRES-Tomato MEF2C

    Plasmid

    #89715

    Purpose

    Retroviral gene expression vector for MEF2C expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Spike Display_B.1.1.7

    Plasmid

    #172735

    Purpose

    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. Spike Display_B.1.1.248

    Plasmid

    #172737

    Purpose

    Expression vector for SARS-CoV-2 (B.1.1.248) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.1.248 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. Spike Display_B.1.351

    Plasmid

    #172736

    Purpose

    Expression vector for SARS-CoV-2 (B.1.351) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.351 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. Spike Display_B.1.427/B.1.429

    Plasmid

    #172738

    Purpose

    Expression vector for SARS-CoV-2 (B.1.427/B.1.429) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.427/B.1.429 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 8, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge