We narrowed to 26,511 results for: RON

1. pLVPRT-tTR-KRAB

   Plasmid

   #11648

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hPrion, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGG>nls-hCas9-nls

   Plasmid

   #99138

   Purpose

   Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   humanized Cas9
   

   Use

   CRISPR

   Promoter

   Chicken beta actin (CAG)

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5KO9 (HMCES)

   Plasmid

   #101877

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HMCES (HMCES Human)
   

   Tags

   6x His (modified C-term tag is AENLYFQSHHHHHH)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. 4IKP

   Plasmid

   #60080

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CARM1:JMC049-A06:C226836 (CARM1 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis

   Mutation

   See comments

   Promoter

   polyhedrin

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pLVPT-rtTR-KRAB

   Plasmid

   #11777

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, rtTR-KRAB, Tet-off
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. JMC044-B12

   Plasmid

   #58752

   Purpose

   bacterial expression of PDB 4NW3

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4NW3 (KMT2A Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   Aug. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pJH1579

   Plasmid

   #178904

   Purpose

   Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60

   Depositor

   Mei Zhen

   Article

   Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

   Insert

   YC3.60
   

   Expression

   Worm

   Mutation

   See Depositor Comments Below

   Promoter

   Pglr-1

   Available Since

   Jan. 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 6BHD (SETDB1)

   Plasmid

   #119955

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1 (SETDB1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 190-410

   Promoter

   T7

   Available Since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pICH51288

   Plasmid

   #50269

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. ii-Str_SBP-mCherry-GPI

    Plasmid

    #65297

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Syt1 C2AB (96-421) D303G

    Plasmid

    #170646

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D303G

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX Syt1 C2AB (96-421) D365E

    Plasmid

    #170649

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D365E

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Syt1 C2AB (96-421) I367T

    Plasmid

    #170652

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    I367T

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5T5G (SETD8)

    Plasmid

    #101886

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD8 (KMT5A Human)
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pBT373

    Plasmid

    #125614

    Purpose

    Expresses FERNY-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    FERNY-BE4max-NG
    

    Expression

    Mammalian

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-AP.Baz1a.1-GFP-WPRE

    Plasmid

    #211490

    Purpose

    expresses GFP under artificial promoter Baz1a

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOGG026

    Plasmid

    #113992

    Purpose

    Level 1 golden gate cloning vector for low copy number stable environmental gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-AP.Agmat.1-GFP-WPRE

    Plasmid

    #211489

    Purpose

    expresses GFP under artificial promoter Agmat

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b-ecDHFR

    Plasmid

    #109055

    Purpose

    Bacterial expression of E.coli DHFR protein

    Depositor

    Stephen Benkovic

    Article

    Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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