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We narrowed to 11,245 results for: ENA

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Showing: 10651 - 10700 of 11245 results

  2. pFAB1902

    Plasmid
    #47858
    Purpose
    BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    M13_central_T_linker_rrnD_T1 double terminator
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    concatenate of M13 central and rrnD
    Promoter
    pLTetO13
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47858-plasmid-map-sequence-id-68390

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  4. pTARGEX-VAC

    Plasmid
    #242450
    Purpose
    Targets high levels of recombinant protein to vacuole. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    C-terminal propeptide (CTPP) derived from N. taba…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242450-plasmid-map-sequence-id-489544

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  6. FLuc-C-HiBiT

    Plasmid
    #207118
    Purpose
    Bacterially expressed FLuc-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    luciferin 4-monooxygenase
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207118-plasmid-map-sequence-id-417161

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  12. pASPIre4

    Plasmid
    #196656
    Purpose
    Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
    Expression
    Bacterial
    Mutation
    SpeI site in Bxb1 CDS
    Promoter
    rhamnose-inducible promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196656-plasmid-map-sequence-id-403168

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  24. iEscSnFR

    Plasmid
    #182807
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iEscSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182807-plasmid-map-sequence-id-358170

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  25. iFluoxSnFR

    Plasmid
    #182808
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iFluoxSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182808-plasmid-map-sequence-id-358173

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  31. pCIS-rPALcc

    Plasmid
    #145804
    Purpose
    expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells
    Depositor
    Betty Eipper, Richard Mains
    Article
    Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9
    Insert
    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    Expression
    Mammalian
    Mutation
    changed serine 767 to alanine to prevent glycosyl…
    Promoter
    CMV
    Available Since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    145804-plasmid-map-sequence-id-299336

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  32. iSKetSnFR2

    Plasmid
    #140467
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR2
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140467-plasmid-map-sequence-id-296110

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  33. iSKetSnFR1

    Plasmid
    #140466
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR1
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140466-plasmid-map-sequence-id-296106

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  36. pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H

    Plasmid
    #108378
    Purpose
    FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108378-plasmid-map-sequence-id-209430

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  37. pcDNA5/FRT/TO-TPI_WT-xrRNA-4H

    Plasmid
    #108377
    Purpose
    FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108377-plasmid-map-sequence-id-209424

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  46. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid
    #234724
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.
    Depositor
    Kirill Martemyanov
    Article
    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234724-plasmid-map-sequence-id-466565

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Showing: 10651 - 10700 of 11245 results