8,917 results

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pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-hGSDME

Plasmid

#154876

Purpose

Expresses hGSDME in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

Lentiviral

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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1099 pcDNA GFP FKHR AAA

Plasmid

#9023

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-AHPH-DM

Plasmid

#71368

Purpose

Location sensor for RhoA-GTP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

C terminus of Anillin (ANLN Human)

Tags

EGFP

Expression

Mammalian

Mutation

A740D and E758K

Promoter

CMV

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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GAPDH-CPG-TV

Plasmid

#70148

Purpose

a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
SNX9-pmCherryC1

Plasmid

#27678

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

SNX9 (Snx9 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pHLUM

Plasmid

#40276

Depositor

Markus Ralser

Article

Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

Inserts

LEU2 (LEU2 Budding Yeast)
URA3 (URA3 Budding Yeast)
MET15/17 (MET17 Budding Yeast)

Expression

Yeast

Promoter

endogenous LEU2, endogenous MET15/17, and endogen…

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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m168

Plasmid

#34886

Depositor

Irvin Chen

Article

Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

Insert

Sindbis virus envelope protein

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas G12V

Plasmid

#18666

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

G12V mutation in HRas

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-Flag-PALB2

Plasmid

#71114

Purpose

mammalian expession of FLAG-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pClneoMyc-LATS1

Plasmid

#66851

Purpose

Express Myc-tagged LATS1 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS1 (LATS1 Human)

Tags

Myc

Expression

Mammalian

Mutation

P44L mutation in LATS1 compared to GenBank refere…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCA-mTmG

Plasmid

#26123

Depositor

Liqun Luo

Insert

Precursor mT/mG

Tags

membrane tag

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-HA-GFP

Plasmid

#22612

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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LZ10 PBREBAC-H2BHalo

Plasmid

#91564

Purpose

piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

Depositor

James Zhe Liu

Article

Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

Insert

histone H2B-HaloTag

Tags

HaloTag

Expression

Mammalian

Promoter

EF1

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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