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Showing: 6421 - 6450 of 8073 results

  3. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  11. Avr2-GFP pZK538

    Plasmid
    #118011
    Purpose
    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .
    Depositor
    FLW Takken
    Article
    et al Bio-protocol 8(24): e3114.
    Inserts
    dspAvr2
    mCherry
    Use
    Tags
    EGFP and HDEL
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    35S
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    118011-plasmid-map-sequence-id-307579

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  12. EPHA7 (3NRU)

    Plasmid
    #28126
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPHA7:SDC173-D03:C209131 (EPHA7 Human)
    Use
    Tags
    His and Honeybee melittin Signal sequence (MKFLVN…
    Expression
    Bacterial
    Mutation
    contains amino acids residues 32-204.
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pCoofy62

    Plasmid
    #122014
    Purpose
    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122014-plasmid-map-sequence-id-243584

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  14. pEGFP-(RTRG)6

    Plasmid
    #69849
    Purpose
    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.
    Insert
    6 RTRG repeats
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69849-plasmid-map-sequence-id-125695

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  15. pET28a-λN-βLac

    Plasmid
    #162039
    Purpose
    Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)
    Depositor
    Karen Polizzi
    Article
    Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199
    Insert
    λN domain of the λ phage and a β-lactamase fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162039-plasmid-map-sequence-id-325526

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  18. nucHCB2

    Plasmid
    #98581
    Purpose
    Nuclear Huntingtin Chromobody 2: "iVHH4" intrabody recognizing amino acids 49–148 of huntingtin, fused to eYFP and nuclear localization signal.
    Depositor
    Ray Truant
    Article
    Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.
    Insert
    HCB2
    Use
    Tags
    NLS and YFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    98581-plasmid-map-sequence-id-189303

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  23. Rev7-Halo HRD

    Plasmid
    #207079
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous REV7 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human REV7 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    207079-plasmid-map-sequence-id-471050

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  24. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207082-plasmid-map-sequence-id-430992

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  25. pCAG-memRFP

    Plasmid
    #224564
    Purpose
    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    Unspecified
    Tags
    Membrane localization signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224564-plasmid-map-sequence-id-451482

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  28. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid
    #216307
    Purpose
    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216307-plasmid-map-sequence-id-433370

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  30. Rif1-Halo HRD

    Plasmid
    #207081
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207081-plasmid-map-sequence-id-432324

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Showing: 6421 - 6450 of 8073 results