We narrowed to 34,448 results for: CaS;

1. pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

   Plasmid

   #223152

   Purpose

   Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1b (CBX1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 79-173

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

   Plasmid

   #209783

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   miR122 ts
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

   Plasmid

   #196828

   Purpose

   Express dpdpCas13b with AAAA mutation

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Use

   Tags

   3XFLAG and NES

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

   Plasmid

   #189633

   Purpose

   Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

   Plasmid

   #211819

   Purpose

   lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   SpCas9, tracrRNA and blasticidin resistance gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a promoter and miniU6 promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

   Plasmid

   #189632

   Purpose

   Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

   Plasmid

   #205417

   Purpose

   Mutagenesis of Ntsr1, second guide

   Depositor

   Larry Zweifel

   Insert

   Ntsr1 (Ntsr1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

   Plasmid

   #200124

   Purpose

   Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human DsRed-Monomer CASP9 C287A Mutant

    Plasmid

    #198469

    Purpose

    Expresses Human DsRed-Monomer CASP9 C287A Mutant Fusion Protein in Mammalian Cells

    Depositor

    Hannah Rabinowich

    Insert

    Human Caspase-9 C287A Mutant (CASP9 Human)
    

    Use

    Tags

    DsRed-monomer

    Expression

    Mammalian

    Mutation

    Changed Cysteine 287 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. N-terminal 3X Flag Human CASP9 C287A Mutant

    Plasmid

    #198380

    Purpose

    Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in Mammalian Cells

    Depositor

    Hannah Rabinowich

    Article

    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

    Insert

    N-Terminal 3X Flag Human Caspase-9 C287A Mutant (CASP9 Human)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 287 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196081

    Purpose

    Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196083

    Purpose

    Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196075

    Purpose

    Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196077

    Purpose

    Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

    Plasmid

    #190903

    Purpose

    AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    GFP, sgHTT and sgCas9 (HTT Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-Inducible Mouse C325A Caspase-9 Mutant

    Plasmid

    #194500

    Purpose

    Inducible Expression of Mouse C325A Caspase-9 Mutant

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    Mouse Caspase-9 C325A Mutant (Casp9 Mouse)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

    Plasmid

    #192131

    Purpose

    Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

    Plasmid

    #192133

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-KY
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (K390A, Y446A)

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

    Plasmid

    #192132

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-Y
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (Y446A)

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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