-
Plasmid#238038PurposeEncodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterlacAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
BtARR1 R175E
Plasmid#236263PurposeBacterial expression of bovine arrestin-1 R175E with inserted GGT codon after starting ATGDepositorInsertarrestin-1 (SAG Bovine)
UseTagsExpressionBacterialMutationR175E, inserted GGT codon after starting ATGPromoterlacAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs N-terminal sgRNA
Plasmid#207093PurposepX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.DepositorInsertAGCGGGACTCGGCGGCATGG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-PCNAL2 (pc3385)
Plasmid#213140PurposeExpresses human PCNA miRFP670 tagged in mammalian cellsDepositorInsertPCNA (PCNA Human)
UseTagsmiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV[FLEX]-CAG-EGFP-Ctrl-sponge
Plasmid#235152PurposeExpresses a EGFP-based control sponge in a Cre recombinase-dependent mannerDepositorInsertEGFP/control sponge
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DD-T6B-WT-YFP
Plasmid#235143PurposeExpresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoterDepositorInsertDHFR-fused T6B peptide
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl
Plasmid#235158PurposeExpresses EGFP and a control primary transcript in a Cre recombinase-dependent mannerDepositorInsertEGFP/primary transcript with control siRNA
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-L7-6-DD-T6B-WT-YFP
Plasmid#235145PurposeExpresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoterDepositorInsertDHFR-fused T6B peptide
UseAAVTagsExpressionMutationPromoterL7-6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-L7-6-FLAG-HA-T6B-WT-YFP
Plasmid#235141PurposeExpresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoterDepositorInsertT6B peptide
UseAAVTagsFLAG/HAExpressionMutationPromoterL7-6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLAG-HA-T6B-WT-YFP
Plasmid#235139PurposeExpresses the T6B peptide fused with YFP under the human synapsin promoterDepositorInsertT6B peptide
UseAAVTagsFLAG/HAExpressionMutationPromoterhSynAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA4TO_24xGCN4_PCC_24xPP7
Plasmid#236094PurposeConstitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.DepositorInsert24xGCN4-PCC-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO_24xGCN4_MUT_24xPP7
Plasmid#236093PurposeConstitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.DepositorInsert24xGCN4-MUT-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_GFP
Plasmid#235611PurposeEncodes GFP. For use in transfections as a negative control without epitope tags.DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_GFP
Plasmid#235632PurposeEncodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.DepositorInsertGFP
UseTagsNES-V5ExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPorig
Plasmid#221092PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SUMO-LS4
Plasmid#228450PurposeLS4 protein expression vector for bacteriaDepositorInsertLS4
UseTags10xHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SUMO-LS12
Plasmid#228451PurposeLS12 protein expression vector for bacteriaDepositorInsertLS12
UseTags10xHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVEX2.3-CS1M2b-3c-ON8-EGFP
Plasmid#228452PurposeLS4-responsive riboswitchDepositorInsertCS1M2b-3c-ON8 riboswitch-regulated EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVEX2.3-CS2-3c-ON8-EGFP
Plasmid#228453PurposeLS12-responsive riboswitchDepositorInsertCS2-3c-ON8 riboswitch-regulated EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-NFAPorig
Plasmid#221091PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-NFAPorig
Plasmid#221086PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-CFAPorig
Plasmid#221087PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-mir302-8mer4x (pEZ185)
Plasmid#229059PurposeDual-luciferase reporter containing 4 copies of the 8mer site of human miR-302aDepositorInsertHuman miR-302a binding sites
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NLS-HA (pKW919)
Plasmid#229047PurposeMammalian expression of CasRx-NLS-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NES-HA (pKW387)
Plasmid#229048PurposeMammalian expression of CasRx-NES-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNES-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-DEST (JDW 1205)
Plasmid#229821PurposeA PiggyBac destination vector compatible with gateway cloning system.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-DEST (JDW 940)
Plasmid#229825PurposeA neo selectable, PiggyBac destination vector compatible with gateway cloning system.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-phiC31o (JDW 453)
Plasmid#229842PurposeA CMV driven Phi31 recombinase.DepositorInsertphiC31 codon optimized, PhiC31o
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif1A-EGFP-SNAP
Plasmid#229851PurposeKif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif1A-EGFP-SNAP (Kif1a Rat)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJH4040
Plasmid#229803PurposePgpc-1 wCherry unc-54 3' UTR C.elegans neuron and other structures expression of RFPDepositorInsertPgpc-1 wCherry unc-54 3' UTR
UseTagsRFPExpressionWormMutationPromoterPgpc-1Available sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430
Plasmid#221726PurposeExpression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.DepositorInsertBleoR-miR-430
UseFish expressionTagsExpressionMutationPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH-GFPminimizer
Plasmid#202488PurposeExpresses the nanobody GFP minimizer with T7 inductionDepositorInsertnanobody GFP minimizer
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH72
Plasmid#202467PurposeExpresses the nanobody VHH72 after phosphate depletionDepositorInsertnanobody VHH72
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-H6antivenin
Plasmid#202470PurposeExpresses the nanobody H6 antivenin after phosphate depletionDepositorInsertnanobody H6 antivenin
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-1B5
Plasmid#202472PurposeExpresses the nanobody 1B5 after phosphate depletionDepositorInsertnanobody 1B5
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-2E7
Plasmid#202473PurposeExpresses the nanobody 2E7 after phosphate depletionDepositorInsertnanobody 2E7
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-3E3
Plasmid#202474PurposeExpresses the nanobody 3E3 after phosphate depletionDepositorInsertnanobody 3E3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-C9BoNT-A
Plasmid#202475PurposeExpresses the nanobody C9 BoNT-A after phosphate depletionDepositorInsertnanobody C9 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-E7BoNT-A
Plasmid#202476PurposeExpresses the nanobody E7 BoNT-A after phosphate depletionDepositorInsertnanobody E7 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only