We narrowed to 11,588 results for: EMB
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Plasmid#169016PurposeExpresses GST-UBXN2A (human) in insect cellsDepositorAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP
Plasmid#168491PurposeMammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertthe E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r
Plasmid#162956PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHis6-Halotag fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r
Plasmid#162934PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoterDepositorInsertHis6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r
Plasmid#162933PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoterDepositorInsertHis6-maltose binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-MIOX only
Plasmid#166683PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertGFP-MIOX
UseSynthetic BiologyExpressionYeastPromoterGAL10Available SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
wtVP1 only
Plasmid#166673PurposeYeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertWild-type Murine polyomavirus VP1
UseSynthetic BiologyExpressionYeastPromoterGAL1Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)
Plasmid#160582Purpose35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)DepositorInsertattR:P35S:TMtb:attL
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 TNos:Luc:5'UTR (GB1499)
Plasmid#160580PurposeReversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoterDepositorInsertLuc
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 linker-RDF (GB2892)
Plasmid#160604PurposeRDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequenceDepositorInsertRDF
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-S11-GFP1–9
Plasmid#125666PurposeBinary vector for the inducible expression of ER-targeted GFP1–9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertER-S11-GFP1–9
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R373
Plasmid#138243PurposeExpresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XbaI-p15A-aadA
Plasmid#149338Purposeexpresses M.XbaI DNA methyltransferase in E. coliDepositorInsertM.XbaI
ExpressionBacterialPromoterrhamnoseAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R362
Plasmid#138232PurposeExpresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R376
Plasmid#138246PurposeExpresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R363
Plasmid#138233PurposeExpresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R366
Plasmid#138236PurposeExpresses the split mCherry reporter interrupted by the LLP-Pol intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R374
Plasmid#138244PurposeExpresses the split mCherry reporter interrupted by the SaP-Hel artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R355
Plasmid#138225PurposeExpresses the split mCherry reporter interrupted by the Cro-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R356
Plasmid#138226PurposeExpresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R357
Plasmid#138227PurposeExpresses the split mCherry reporter interrupted by the Cro-Top2 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R358
Plasmid#138228PurposeExpresses the split mCherry reporter interrupted by the CIV-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R361
Plasmid#138231PurposeExpresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Rab28_Anti-Sense
Plasmid#124442PurposePlasmid for anti-sense in situ probe in vitro transcriptionDepositorAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SA](315)
Plasmid#122073PurposeExpressing GFP-Atg13[3SA]DepositorInsertATG13
TagsGFPExpressionYeastMutationSerine 646 to alanine; Serine 649 to alanine; Ser…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CURT_flouB
Plasmid#117995PurposeExpression of CURT_flouB controlled by the CaMV 35S promoterDepositorInsertCURT_flouB
UseYeast/bacteria binary vectorExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFL His-I3 V41A / W43A
Plasmid#113515PurposeExpression of His-I3 V41A / W43 A in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialPromoternativeAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E9S7M7
Plasmid#103106PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE9S7M7(Cas9 coding gene from Ruminococcus albus 8)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mCherry Gamma 3
Plasmid#65234PurposeTo study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cellDepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Triple mutant
Plasmid#63719PurposeVoltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227DDepositorInsertTriple mutant
TagsSuper ecliptic pHlorin A227DExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1522
Plasmid#63794PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3 selection markerDepositorInserteVenus-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseVenus-Twin-StrepPromoternoneAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1422
Plasmid#63793PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection markerDepositorInserteCitrine-Twin-tag followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Twin-tagPromoternoneAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1122
Plasmid#63790PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection markerDepositorInserteSapphire-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseSapphire-Twin-Strep tagPromoternoneAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
NKP91 GFP (1034)
Plasmid#62023Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hPMCA4b-D672E
Plasmid#47596DepositorInsertPMCA4b-D672E (ATP2B4 Human)
ExpressionMammalianMutationD672E (reducing the ATPase activity to less than…PromoterCMVAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
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TAL-BBL1-ID14
Plasmid#41516DepositorInsertPolylinker flanked by 2 Mva1269I cut sites
ExpressionMammalianPromoterSV40Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL-BBL1-ID43
Plasmid#41517DepositorInsertPolylinker flanked by 2 Mva1269I cut sites
ExpressionMammalianPromoterSV40Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL-BBL1-ID32
Plasmid#41518DepositorInsertPolylinker flanked by 2 Mva1269I cut sites
ExpressionMammalianPromoterSV40Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL-BBL1-ID21
Plasmid#41519DepositorInsertPolylinker flanked by 2 Mva1269I cut sites
ExpressionMammalianPromoterSV40Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TALEN-ID12-A
Plasmid#41476DepositorInsertTALEN-FokI nuclease backbone
Tags3xFLAG and WT FokI nuclease domainExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TALEN-ID12-G
Plasmid#41478DepositorInsertTALEN-FokI nuclease backbone
Tags3xFLAG and WT FokI nuclease domainExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
GIMAP1 (3LXW)
Plasmid#25336PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGIMAP1:E25-R253 (GIMAP1 Human)
TagsHisExpressionBacterialMutationContains amino acids E25-R253Available SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Xwnt2b-pXT7
Plasmid#32988DepositorAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1133
Plasmid#29054PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pGSTag BimL(AA)
Plasmid#25062DepositorInsertBimL (AA) (BCL2L11 Human)
TagsGstExpressionBacterialMutationChanged threonine 56 to alanine Changed serine 5…Available SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
GIMAP4
Plasmid#25337PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGIMAP4:G20-M240 (GIMAP4 Human)
TagsHisExpressionBacterialMutationContains amino acids G20-M240Available SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3 T7 BimL (S44D)
Plasmid#24530DepositorInsertBim L(S44D) (BCL2L11 Human)
TagsT7ExpressionMammalianMutationChanged serine 44 to aspartic acidAvailable SinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only