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pEGB 35S:DsRed:Tnos (GB0361)

Plasmid

#68220

Purpose

Transcriptional unit for DsRed plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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GST-N1IC

Plasmid

#47612

Purpose

bacterial expression of GST-myc-Notch1 IC

Depositor

Urban Lendahl

Article

Beatus et al Development. 1999 Sep;126(17):3925-35.

Insert

Notch1 intracellular domain (Notch1 Mouse)

Tags

GST and myc

Expression

Bacterial

Mutation

contains amino acids 1753-2531

Promoter

tac

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
RAD Module G8-C1

Plasmid

#38208

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

SET Integrase
Bxb1 Excisonase
RESET Integrase

Use

Synthetic Biology; Biobrick cloning vector

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (∂393)

Plasmid

#24812

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-393. (No PDZ binding…

Available Since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA 3.1 AnxA6

Plasmid

#20407

Depositor

Carlos Enrich

Article

Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

Insert

AnxA6 (Anxa6 Rat)

Expression

Mammalian

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

Plasmid

#162877

Purpose

Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)

Tags

mEos2

Expression

Mammalian

Mutation

Insert consists of AA1-170

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP10_nostop

Plasmid

#149313

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP4_nostop

Plasmid

#149307

Purpose

Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pQCXIP-GFP-RPA70

Plasmid

#164231

Purpose

retroviral plasmid for GFP-RPA70

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

RPA70 (RPA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 19

Plasmid

#134987

Purpose

Expression of human Myo 19 in mammalian cells.

Depositor

Martin Bähler

Article

Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

Insert

Myo 19 (MYO19 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMEV4

Plasmid

#110539

Purpose

The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

Depositor

William B. Whitman

Article

Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pMPRA2

Plasmid

#49350

Purpose

Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAT206/Sept7shRNA

Plasmid

#38299

Depositor

Matthew Krummel

Article

Tooley et al Nat Cell Biol. 2009 Jan;11(1):17-26. Epu

Insert

Septin 7 (Septin7 Mouse)

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-C1C2-TS-EYFP

Plasmid

#35520

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

Trafficking signal and EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Rnd2

Plasmid

#23228

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd2 (RND2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
GFP-RhoH

Plasmid

#23230

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoH (RHOH Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pQCXIB empty (w335-1)

Plasmid

#17487

Purpose

Retroviral Empty control vector, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PL-452 C-YPet

Plasmid

#19177

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

YPet

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
MMTV-GAL4-SV40

Plasmid

#19255

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Insert

GAL4

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
Bnip3L RNAi pSuper-retro

Plasmid

#17468

Depositor

Wafik El-Deiry

Article

Fei et al Cancer Cell. 2004 Dec . 6(6):597-609.

Insert

Bnip3L RNAi (BNIP3L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only