We narrowed to 11,726 results for: pcdna

1. pcDNA5/FRT/TO CBX1-APEX2

   Plasmid

   #129642

   Purpose

   Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   CBX1 (CBX1 Human)
   

   Use

   Tags

   1xFLAG and APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-Beclin1-(151-241)

   Plasmid

   #24393

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acids 151-241 are deleted. Delta CC mutant.

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. hE-cadherin/α-catenin fusion-pcDNA3

   Plasmid

   #45771

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Frog, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

   Promoter

   CMV

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-mas-KB-1753-Nluc

   Plasmid

   #158603

   Purpose

   Plasmid expressing KB-1753-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Yellow Cameleon-Nano140/pcDNA3

   Plasmid

   #51966

   Purpose

   mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano140
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT-Flag-Cul5

   Plasmid

   #31984

   Purpose

   Mammalian expression of Flag-tagged Cul5

   Depositor

   Joan Conaway

   Article

   Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

   Insert

   Cul5 (CUL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcdna6.2- myc ULK1 k46I

   Plasmid

   #27630

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2010 Dec 23. ():.

   Insert

   mouse myc ULK1 K46I (Myc Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Promoter

   Available since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1 CMV NAPA-A BGH

   Plasmid

   #92277

   Purpose

   FRET probe; extracellular membrane tethered eGFP

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   Neuron-astrocyte proximity assay - Astrocyte
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO-FNTA-FLAG

   Plasmid

   #86858

   Purpose

   To express FNTA with a FLAG tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTA (FNTA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The sequence of the insert is isoform 2, which di…

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1307 pcDNA3 GFP PTEN A4

    Plasmid

    #10760

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A4: S380A, T382A, T383A, S385A

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

    Plasmid

    #181850

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-hRIPK1-Flag-delN

    Plasmid

    #112492

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of N terminus (AA1-274 deleted)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid

    #112493

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of C terminus (AA290-671 deleted)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2/V5 mmu-mir-133a-1

    Plasmid

    #26326

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-133a-1 (Mir133a-1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1(+)/CUG-nLuc-3XFLAG

    Plasmid

    #127300

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO HIS DNAJB1

    Plasmid

    #19549

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1 myc-Rab10

    Plasmid

    #208366

    Purpose

    Mammalian expression of myc-tagged human Rab10

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    myc-Rab10 (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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