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Plasmid#191355PurposeClostridium expression vector with TetR-repressed NanoLuc expressionDepositorInsertPGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette
UseTagsExpressionBacterialMutationPromoterPGus2A-TetO2/1Available sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP
Plasmid#103815PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
Plasmid#149426PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pISH-Adrb2
Plasmid#105199Purposeto generate a riboprobe for in situ hybridazationDepositorInsertAdrb2 (Adrb2 Mouse)
UseTo ganerate riboprobeTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-Venus-Sec61β
Plasmid#174467PurposeExpress Venus with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-EGFP
Plasmid#175839PurposeExpress EGFP with nucleus-targeting sequence in mammalian cellsDepositorInsertEGFP with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-mCherry
Plasmid#103816PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut
Plasmid#42040DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa457-532, aa716-772 and aa804-924, F951A…PromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-Sec61β
Plasmid#174468PurposeExpress mCherry with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
E0030 (YFP)_CD
Plasmid#66031PurposeMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]DepositorInsertFluorescent reporter - YFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
E1010m (RFP)_CD
Plasmid#66033PurposeMoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]DepositorInsertFluorescent reporter - RFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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KTK_136
Plasmid#180520PurposeEmpty backbone for D1.1 cloning, contains sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCmR1.1
Plasmid#59014PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N10_trigger
Plasmid#132728PurposeT7 RNAP-driven expression of 3WJ repressor index 10 trigger RNADepositorInsert3WJ_N10_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N10_switch
Plasmid#132724PurposeT7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N10_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-SECFP
Plasmid#174470PurposeExpress SECFP with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertSECFP with golgi apparatus-targeting sequence
UseTagsβ1,4-galactose transferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-SECFP
Plasmid#174185PurposeExpress SECFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertSECFP with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110561PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertstRBS-aacC1
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-iRFP
Plasmid#174474PurposeExpress iRFP with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertiRFP with golgi apparatus-targeting sequence
UseTagsβ1,4-galactose transferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIW086
Plasmid#134132PurposeBacterial expression of His-TEV-Ska1 and Ska2DepositorInsertHis-TEV-human Ska1 ; human Ska2
UseTagsHis-TEVExpressionBacterialMutationK183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIhh-5A9-LUC
Plasmid#46318DepositorInsert5 copies of A9 sequence from Ihh promoter
UseTagsluciferaseExpressionMammalianMutationPromotermouse osteocalcin gene 2 promoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
KTK_001
Plasmid#180517PurposeEmpty backbone for creation of entry parts for the KTK, contains sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVTagsExpressionMammalianMutationPromoterEF1α promoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N11_trigger
Plasmid#132733PurposeT7 RNAP-driven expression of 3WJ repressor index 11 trigger RNADepositorInsert3WJ_N11_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969PromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716PromoterAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
E0040m (GFP)_CD
Plasmid#66032PurposeMoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D]DepositorInsertFluorescent reporter - GFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KTK_247
Plasmid#180529PurposeEntry vector containing pTac promoterDepositorInsertpTac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
TA0474_pET21a(+)
Plasmid#13839DepositorInsertIS200 transposase (Ta0474 Thermoplasma acidophilum)
UseTagsExpressionBacterialMutationSee Depositor Comments BelowPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-CXCR4
Plasmid#42052DepositorInsertCXCR4 shRNA (CXCR4 )
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP
Plasmid#103817PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP (CRY2 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_AE
Plasmid#66063PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIhh1.3K-Luc
Plasmid#53604PurposeReporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-Venus
Plasmid#174472PurposeExpress Venus with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertVenus with golgi apparatus-targeting sequence
UseTagsβ1,4-galactose transferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-Venus
Plasmid#174187PurposeExpress Venus with mitochondria-targeting sequence in mammalian cellsDepositorInsertVenus with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
B0032m_BC
Plasmid#66020PurposeMoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_EF
Plasmid#66064PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pIhh715bp-Luc
Plasmid#53606PurposeReporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
beta4-nAChR (DPM negative control)
Plasmid#86651Purposeexpression of control shRNADepositorInsertCHRNB4 (control)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cwc25-pRSETA
Plasmid#61043PurposeEncodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.DepositorInsertCwc25
UseTagsTEV site, Histidine TagExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin (1-76; L71Y)
Plasmid#110756PurposeBacterial expression of human Ubiquitin (1-76; L71Y)DepositorInsertUBB (UBB Human)
UseTagsExpressionBacterialMutationL71YPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA4b delta6
Plasmid#47590DepositorInsertPMCA4bdelta6 (ATP2B4 Human)
UseTagsEGFPExpressionMammalianMutation6 C-terminal AA deleted (needed for PDZ binding)PromoterCMVAvailable sinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
mproSAAS (21 kDa)
Plasmid#231634PurposeMammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)DepositorInsertPcsk1N (Pcsk1n Mouse)
UseTagsExpressionMammalianMutationDeletion of C-terminus (aa181-225)PromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)
Plasmid#205761PurposeSingle-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transitionDepositorInsertrsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only