We narrowed to 5,541 results for: SPI

1. pcDNA3-J chain

   Plasmid

   #145146

   Purpose

   Mammalian expression plasmid for J chain of polymeric antibody classes

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   immunoglobulin J chain (JCHAIN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSicoR-Ef1a-mCh

   Plasmid

   #31847

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBEI-6410

   Plasmid

   #47049

   Purpose

   BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
   

   Use

   Synthetic Biology; Bglbrick

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacUV5

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF160_VSV-G

   Plasmid

   #225963

   Purpose

   CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-VSVG (env protein)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES2Cx32WTEGFP

   Plasmid

   #78150

   Purpose

   Mammalian Expression of Connexin 32 and EGFP from a bicistronic mRNA

   Depositor

   Mario Bortolozzi

   Article

   Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

   Insert

   Connexin 32 (GJB1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172318

   Purpose

   CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. aMHC-mCherry-Rex-Blasticidin

   Plasmid

   #21228

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGY577

   Plasmid

   #166663

   Purpose

   Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

   Depositor

   Gaël Yvert

   Article

   Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

   Insert

   LiCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cre: E340A, D341A

   Promoter

   CMV

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-Kif2A

   Plasmid

   #52401

   Purpose

   Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

   Depositor

   Gohta Goshima, Ryota Uehara

   Article

   Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

   Insert

   Kif2A (KIF2A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. HeLa full-length MLCK-GFP

    Plasmid

    #46316

    Depositor

    Anne Bresnick

    Article

    Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

    Insert

    HeLa long myosin light chain kinase (MYLK Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. aMHC-eGFP-Rex-Neo

    Plasmid

    #21229

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cox7c-pHLuorin-sems

    Plasmid

    #107766

    Purpose

    labeling of mitochondrial cytochrome c oxidase, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit Cox7c fused to pHluorin (COX7C Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204188

    Purpose

    Doxycycline inducible lentiviral vector for human NOTUM overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NOTUM (NOTUM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CYFIP1.mCherry

    Plasmid

    #122051

    Purpose

    Expresses human CYFIP1 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PR_GEF YFP (2XPDZ-YFP-LARG DH)

    Plasmid

    #80408

    Purpose

    Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

    Depositor

    Michael Glotzer

    Article

    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

    Insert

    2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. OTR untagged

    Plasmid

    #208913

    Purpose

    encodes the human oxytocin receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    OXTR (OXTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TM5: 5x56, A218, Threonin instead of Alanin

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid

    #89637

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hela kinase-dead MLCK-GFP

    Plasmid

    #46317

    Depositor

    Anne Bresnick

    Article

    Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

    Insert

    HeLa long myosin light chain kinase-dead (MYLK Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    E1626K

    Promoter

    CMV

    Available since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. SARS_COV2_ORF10_HA_BASU

    Plasmid

    #153980

    Purpose

    Express Orf10 gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dCpf1-eBE

    Plasmid

    #107688

    Purpose

    Expresses dLbCpf1-eBE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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