We narrowed to 9,202 results for: sacs

1. pCfB2803

   Plasmid

   #63646

   Purpose

   pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-JNKAR1-NES

   Plasmid

   #84632

   Purpose

   Unimolecular, FRET-based JNK activity reporter; cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ECFP-FHA1-JNKsubDD-Citrine-NES
   

   Use

   Tags

   6xHis, Citrine, ECFP, Nuclear export signal (NES)…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GAL4-mNFAT1(2-144)

   Plasmid

   #11108

   Purpose

   Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

   Depositor

   Anjana Rao

   Article

   Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Use

   Tags

   GAL4-DBD

   Expression

   Mammalian

   Mutation

   Contains NFAT1 amino acids 2-144; L78P and P140Q …

   Promoter

   Available since

   April 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Cerulean-WW-NES

   Plasmid

   #84629

   Purpose

   Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   Cerulean-WW-NES
   

   Use

   Tags

   6xHis, Cerulean, Nuclear export signal (NES), T7 …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p414GPD-3xFLAG-Nop4 WT

   Plasmid

   #169261

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 WT (NOP4 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. BAS292 C terminal SF-GFP kanR

   Plasmid

   #74087

   Purpose

   S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   SF-GFP recoded for S.pombe
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCfB2797

   Plasmid

   #63639

   Purpose

   pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS415-Cas9-VPR

   Plasmid

   #163971

   Purpose

   Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Insert

   Cas9-VPR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDY-HA1-HA2-neo

   Plasmid

   #109123

   Purpose

   FR cassette provider to introduce FR cassette to a BAC by recombineering

   Depositor

   David Gilbert

   Article

   Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

   Inserts

   HA1
   HA2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB2804

    Plasmid

    #63644

    Purpose

    pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

    Plasmid

    #122067

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1-PA (DHH1 Budding Yeast)
    

    Use

    Tags

    PA

    Expression

    Yeast

    Mutation

    Aspartic acid 195 to alanine; Glutamic acid 196 t…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. BAS287 C terminal E2C hygBR

    Plasmid

    #74082

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHES873

    Plasmid

    #87945

    Purpose

    Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB2875

    Plasmid

    #63640

    Purpose

    pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs_FLAG-ODC1

    Plasmid

    #133256

    Purpose

    Express FLAG-tagged ODC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC1 (ODC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. HsSPHK2

    Plasmid

    #118092

    Purpose

    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-pEF1a-DIO-tTAc

    Plasmid

    #172126

    Purpose

    Expression of InteinC-tTAC in Cre recombinase positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-FRT-EBFP-tTAn

    Plasmid

    #172127

    Purpose

    Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    EBFP, tTAn-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET151-Vps4(101-437)

    Plasmid

    #87735

    Purpose

    Bacterial expression of N-terminally truncated S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFA6a-link-GFPIvy-SpHis5

    Plasmid

    #60783

    Purpose

    Yeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selection

    Depositor

    Linda Huang

    Article

    Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

    Insert

    GFPIvy
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    S30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…

    Promoter

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pG4-E6s-luc

    Plasmid

    #46324

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    5xGal4
    

    Use

    Luciferase

    Tags

    E boxes and luciferase

    Expression

    Mammalian

    Mutation

    five copies of a multimerized GAL4 (5×GAL4) site …

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pGST-TEV-yVms1(1-417,delta(38-69))

    Plasmid

    #128739

    Purpose

    Bacterial overexpression and crystallization

    Depositor

    Chris Hill

    Article

    Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

    Insert

    Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 1-417 with deletion of 38-69

    Promoter

    T7

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLPC-MYC.Mm.RAP1.delta.MYB

    Plasmid

    #184660

    Purpose

    Retroviral expression of mouse RAP1 delta MYB

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta MYB

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLPC-MYC.Mm.RAP1.delta.COIL

    Plasmid

    #184659

    Purpose

    Retroviral expression of mouse RAP1 delta COIL

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    pLPC
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta COIL

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLPC-MYC.Mm.RAP1.delta.BRCT

    Plasmid

    #184658

    Purpose

    Retroviral expression of mouse Rap1 delta BRCT

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    RAP1
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta BRCT

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPC-MYC.Mm.RAP1.delta.RCT

    Plasmid

    #184661

    Purpose

    Retroviral expression of mouse RAP1 delta RCT

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    rap1 delta RCT

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUAST mito roGFP2-Orp1

    Plasmid

    #64997

    Purpose

    for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-HJURP-CBP

    Plasmid

    #30393

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    HJURP CenpA Binding Domain (CENPA Budding Yeast, Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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