We narrowed to 14,039 results for: TIM

1. PIK3C2B gRNA (BRDN0001149054)

   Plasmid

   #76712

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PIK3C2B gRNA (BRDN0001149112)

   Plasmid

   #76713

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCX-CD28 WT

   Plasmid

   #223640

   Purpose

   Retroviral expression of mouse CD28 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (Cd28 Mouse)
   

   Use

   Retroviral; mammalian expression

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. VAMP8-mCherry

   Plasmid

   #92424

   Purpose

   Expression of VAMP8 C-terminally conjugated to mCherry.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   VAMP8 (Vamp8 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   A36S (Please see Depositor Comments below)

   Promoter

   CMV

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ1137

   Plasmid

   #84827

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Expression

   Bacterial and Worm

   Promoter

   Peft-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINB-hRNF216

   Plasmid

   #167354

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p415 TEF Su9‐roGFP2‐Tsa2ΔCR

   Plasmid

   #83240

   Purpose

   The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

   Insert

   Su9-roGFP2-Tsa2dCr
   

   Tags

   roGFP2 fusion with Tsa2dCr with Su9 targetting se…

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TNIK gRNA (BRDN0001146045)

   Plasmid

   #75849

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TNIK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TNIK (TNIK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFPC1-ORAI1-EC-HA

   Plasmid

   #174335

   Purpose

   encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   human Orai1 (ORAI1 Human)
   

   Tags

   GFP in the N-terminus and HA tag in the extracell…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRN3P-HA-Suv4-20h2mut

    Plasmid

    #86692

    Purpose

    For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
    

    Tags

    HA

    Mutation

    Mutated asparagine 273 to cysteine 276 (NHDC) int…

    Promoter

    T3

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCFJ1415

    Plasmid

    #84828

    Purpose

    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized GFP
    

    Expression

    Bacterial and Worm

    Promoter

    Peft-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Puro_siKO

    Plasmid

    #86696

    Purpose

    Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-Syn-DIO-TRPV1-P2A-mCherry

    Plasmid

    #200831

    Purpose

    In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-mCherry (Trpv1 Rat)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18T-mini-Tn7T-Gm-Pc-mCherry

    Plasmid

    #199247

    Purpose

    Fluorescent labelling of bacterial cells with mCherry

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized mCherry gene
    

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-ABE6.3

    Plasmid

    #102916

    Purpose

    expresses ABE6.3 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE6.3
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. SiriusGFP

    Plasmid

    #123201

    Purpose

    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

    Insert

    SiriusGFP
    

    Use

    Mouse Targeting

    Tags

    N/A

    Expression

    Bacterial and Mammalian

    Mutation

    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG_Xph20-GCaMP7f

    Plasmid

    #135539

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Tags

    GCaMP7f

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKII-GAL4-iLight-VP16

    Plasmid

    #170274

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
    

    Use

    AAV

    Mutation

    See Comments

    Promoter

    CaMKII

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJR107

    Plasmid

    #187245

    Purpose

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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