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Plasmid#62232PurposeMutant of hamster BiP-mGFP unable to bind clients.DepositorInserthamster BiP-mGFP-KDEL
UseTagsmGFPExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGagMBPHis
Plasmid#42176DepositorInsertGag protein sequence is from HIV-1 clone NL4-3
UseTags6xHIS and MBPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP_rescue_construct
Plasmid#190641PurposePuromycin-selectable expression of GFP in Drosophila S2 cellsDepositorInsertEGFP
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-BFP
Plasmid#190260PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertBFP
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-mCherry
Plasmid#190262PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCherry
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-BFP
Plasmid#190258PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertBFP
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-BFP
Plasmid#190259PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertBFP
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-mCherry
Plasmid#190261PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCherry
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-mScarlet
Plasmid#190263PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRA
Plasmid#108941PurposeMammalian expression of mRIN2 dRADepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseTagsHAExpressionMammalianMutationDeletion of RA domain (aa 751-836)PromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRHVPS9
Plasmid#108942PurposeMammalian expression of mRIN2 dRHVPS9DepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseTagsHAExpressionMammalianMutationDeletion of RHVPS9 domain (aa 455-738)PromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nbr_C_sgRNA2
Plasmid#186664PurposeNbr C-tag sgRNA2 plasmidDepositorInsertNbr sgRNA 2 Plasmid (Nbr Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas9_YTHDF2_sgRNA
Plasmid#186673PurposeYTHDF2 sgRNA plasmidDepositorInsertYTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(Y40C)
Plasmid#164903PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.DepositorInsertRaichuEV-Rac1 (RAC1 Human)
UseTagsExpressionMammalianMutationY40C mutation in the Rac1 domain whcih make the s…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP16 (B1014)
Plasmid#92036PurposeExpresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.DepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 (intact NLS) with VP16 activ…ExpressionMammalianMutationPromoterT7Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Neo
Plasmid#186672PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Neomycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
2C::tdTomato Reporter
Plasmid#40281DepositorInsert2C Promoter (2-cell stage promoter)
UseTagstdTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMJ333: pU6-MmeFz2_omegaRNA-entry
Plasmid#205267PurposeHuman expression vector for MmeFz2 omegaRNA (BbsI guide entry site)DepositorInsertMmeFz2 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ332: pU6-GtFz1_omegaRNA-entry
Plasmid#205265PurposeHuman expression vector for GtFz1 omegaRNA (BbsI guide entry site)DepositorInsertGtFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ106: pRS426-His-MBP-NlovFz2_Native
Plasmid#205253PurposeS. cerevisiae expression vector for NlovFz2 native guideDepositorInsertNlovFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ45: pRS426-His-MBP-SpuFz1_Native
Plasmid#205252PurposeS. cerevisiae expression vector for SpuFz1 native guideDepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ123: pRS426-His-MBP-SpuFz1_PSP1
Plasmid#205256PurposeS. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)DepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM151: pU6-NlovFz2_omegaRNA-entry
Plasmid#205263PurposeHuman expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)DepositorInsertNlovFz2 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-SNAPtag
Plasmid#107372PurposeSynaptic vesicle protein VAMP2 fused to the SNAPtag domainDepositorInsertVAMP2 (Vamp2 Rat)
UseTagsSNAPtagExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ240: pRS426-His-MBP-MmeFz2_Native
Plasmid#205255PurposeS. cerevisiae expression vector for MmeFz2 native guideDepositorInsertMmeFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ235: pRS426-His-MBP-GtFz1_Native
Plasmid#205254PurposeS. cerevisiae expression vector for GtFz1 native guideDepositorInsertGtFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-spGFP(11)
Plasmid#190257PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertspGFP(11)
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only