We narrowed to 1,284 results for: AP2;

1. pCDNA3 Flag MKK4(ala)

   Plasmid

   #15314

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

   Insert

   MKK4(ala) (MAP2K4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-220Ala Thr-224Ala

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

2. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3 Flag MKK7a1 (glu)

   Plasmid

   #14540

   Depositor

   Roger Davis

   Article

   Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

   Insert

   MKK7a1(Glu) (Map2k7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-198Glu Thr-202Glu

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

4. PM2-FRB-CFP

   Plasmid

   #67518

   Purpose

   FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   CFP, HA, and PM tethering sequence from human Lyn…

   Expression

   Mammalian

   Mutation

   FRB domain only (residues 2019–2114)

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-Flag-YAP-S109A

   Plasmid

   #33088

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S109A

   Promoter

   CMV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

6. p21-pRc/CMV

   Plasmid

   #20814

   Depositor

   William Kaelin

   Article

   Adams et al Mol Cell Biol. 1996 Dec . 16(12):6623-33

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGex-6p2_FANCM

   Plasmid

   #221379

   Purpose

   Expresses FANCM in bacterial cells

   Depositor

   Albert Jeltsch

   Article

   Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

   Insert

   Fanconi anemia group M protein (FANCM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLEX-FHH-MTOR HEAT

   Plasmid

   #120709

   Purpose

   Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   MTOR HEAT 1-9 (MTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Amino acids 1-409

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PAmCherry-N1-FRB-Tubbyc

   Plasmid

   #202678

   Purpose

   Photoactivatable and rapamycin recruitable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ps226

    Plasmid

    #133557

    Purpose

    RNAi construct for knock-down of CDKN1A (Cip1, p21)

    Depositor

    David Livingston

    Article

    Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3 Flag MKK4beta

    Plasmid

    #15517

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    pCDNA3 Flag MKK4beta (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3 Flag MKK7a1 (K76A)

    Plasmid

    #14541

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(K76A) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lys-76Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3 Flag MKK7a1 (ala)

    Plasmid

    #14539

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(Ala) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-198Ala Thr-202Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pSVE Grb2 myc 49L/203R

    Plasmid

    #26087

    Depositor

    Bob Weinberg

    Article

    Egan et al Nature. 1993 May 6. 363(6424):45-51.

    Insert

    Grb2 49L/203R (GRB2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    49L/203R

    Promoter

    Available since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAC_N_ELK3

    Plasmid

    #167750

    Purpose

    Expresses ELK3 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

18. sgMTOR_49

    Plasmid

    #125132

    Purpose

    Targeting 49th exon of human MLST8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBG-MKK4 (GST)

    Plasmid

    #21563

    Depositor

    Leonard Zon

    Article

    Sanchez et al Nature. . 372(6508):794-8.

    Insert

    mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pFETCh_MED13_iso1

    Plasmid

    #135733

    Purpose

    Donor vector for 3' FLAG tag of human MED13_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    MED13_iso1 Homology arms (MED13 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pminiT-Halo-MED1 donor

    Plasmid

    #197868

    Purpose

    Donor plasmid used for building Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Halotag

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO-shcIAP1-B

    Plasmid

    #44131

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    c-IAP1 (BIRC2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA FLAG mTOR ST

    Plasmid

    #22999

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pCMV-Flag-YAP-S61A

    Plasmid

    #33087

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A

    Promoter

    CMV

    Available since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

25. PX330_MED13_iso1_1

    Plasmid

    #135751

    Purpose

    Encodes gRNA for 3' target of human MED13_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    MED13_iso1 gRNA (MED13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-2T-FRB-W2027F

    Plasmid

    #73341

    Purpose

    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    

    Use

    Tags

    GST-tagged

    Expression

    Bacterial

    Mutation

    FRB domain only aa2015-2114; W2027F

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pCMV-Flag-YAP-S381A

    Plasmid

    #33090

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S381A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA Flag Yap1 Y357E

    Plasmid

    #18883

    Depositor

    Yosef Shaul

    Article

    Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

    Insert

    Yap1 Y357E (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y357E

    Promoter

    Available since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3 Flag MKK7g1(K165A)

    Plasmid

    #14675

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7g1(K165A) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lys-165Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pGEX-2T FRB Y2105A

    Plasmid

    #68726

    Purpose

    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    

    Use

    Tags

    GST-tagged

    Expression

    Bacterial

    Mutation

    FRB domain only aa2015-2114; Y2105A

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

31. HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)

    Plasmid

    #24653

    Depositor

    Jim Woodgett

    Article

    Yan et al Nature. . 372(6508):798-800.

    Insert

    SEK1 (MAP2K4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Ser220Ala, Thr224Leu

    Promoter

    Available since

    June 21, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pCEP-WAF1-ES

    Plasmid

    #16449

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 stop codon mut (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    stop codon at amino acid 49 instead of tryptophan…

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

33. pCMV-Flag-YAP-H122L

    Plasmid

    #33086

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H122L

    Promoter

    CMV

    Available since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

34. TurboID-MEK1

    Plasmid

    #226169

    Purpose

    Used to transiently express a TurboID fusion of the protein kinase MEK1.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-MEK1 (MAP2K1 Human)
    

    Use

    Tags

    Myc-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGex-6p2_FANCM_K819R

    Plasmid

    #221380

    Purpose

    Expresses FANCM_K819R in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Fanconi anemia group M protein (FANCM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed lysine 819 to arginine

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJM166B

    Plasmid

    #218123

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

    Plasmid

    #214614

    Purpose

    adapter for RTK split TEV recruitment assays

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    3xSH2 domains of GRB2-CTEV (GRB2 Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pminiT-mEmerald-MED1 donor

    Plasmid

    #197867

    Purpose

    Donor plasmid used for building mEmerald-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTij_sg_mmu_Med1_N_terminal

    Plasmid

    #197870

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p8319 pDONR ATG-YAP1 WT-Stop

    Plasmid

    #184524

    Purpose

    Subcloning YAP1 WT

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 WT (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge