We narrowed to 7,699 results for: Lif;
-
Plasmid#207089PurposepX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.DepositorInsertATCATTAAGTACTAGACTCA
ExpressionMammalianPromoterCMV and U6Available SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-muSOX
Plasmid#131701Purposeexpresses muSOX with GFP N-terminally fusedDepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP37
Plasmid#65515PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-Cer-cMycExpressionYeastPromoterpPMA1Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP36
Plasmid#65514PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionYeastPromoterpPMA1Available SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
TagsSNAPExpressionBacterialAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP43
Plasmid#65519PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP39
Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKL13-MgtC bb118
Plasmid#158982PurposeConstitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.DepositorInsertmgtC
ExpressionBacterialPromoterBba_J23118 (sigma 70)Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCO486
Plasmid#139729PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A_35S Short_TMV 5':AsNpga:tAtug7
UseLuciferaseExpressionPlantPromoter35SAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-deltaR8.2(A14C,E45C,A92E)
Plasmid#79048Purposemammalian expression, lentiviral packagingDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
hygII-UT-ABAleon2.15
Plasmid#106983PurposeABAleon2.15 in plant expression vector (Hygromycin resistance)DepositorInsertABAleon2.1
ExpressionPlantPromoterUBQ10Available SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastPromoterpPMA1Available SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastPromoterpPMA1Available SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gag-delta-p6-mEGFP
Plasmid#116890Purposeexpresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cellsDepositorInsertsynthetic Gag missing the p6 domain
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-3H-GFP
Plasmid#205725PurposeExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundleDepositorInsertpOTC-3H-GFP
ExpressionMammalianAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
P366
Plasmid#139722PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A''_pSlRBCS2:AsNpga:tAtuG7
UseLuciferaseExpressionPlantPromoterSlRBCS2Available SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::3xHalo7
Plasmid#87649PurposeFor creating drosophila transgenics expressing UAS-Syt::3xHalo7DepositorInsertSyt::3xHalo7
ExpressionInsectMutationS265G in Syt (please see depositor's commen…Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mVermilion-C1
Plasmid#105756PurposeEmpty vector for creating N terminal mVermilion fusionsDepositorTypeEmpty backboneTagsmVermilionExpressionMammalianAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-QF2
Plasmid#122563PurposeIntegration of QF2 sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-QF2-SV40pA
UseCloningAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx2
Plasmid#104473PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
TagsFKBPx2-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCO487
Plasmid#139730PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, H3HDepositorInsertpMOD_B_AtUbi10:NnH3H:tAtuOcs
UseLuciferaseExpressionPlantPromoterCmYLCVAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCO488
Plasmid#139731PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, HispsDepositorInsertpMOD_C'_CmYLCV:NnHisps:tHsp
UseLuciferaseExpressionPlantPromoterCmYLCVAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGM-ENO2-mCherry-YFP
Plasmid#180570PurposeDual fluorescent reporter for 5' UTR activity in yeast mCherry/YFPDepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) CamKII sf iGluSnFR
Plasmid#158769PurposeiGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.DepositorInsertiGluSnFR
UseLentiviralMutationAdditional ER and Golgi export sequencesAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM-ENO2-YFP-mCherry
Plasmid#180569PurposeDual fluorescent reporter for 5' UTR activity in yeast YFP/mCherryDepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRBx2
Plasmid#104476PurposePlasmid carrying FRBx2 with C-terminal HA tag.DepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_( E3_5)_FLAG
Plasmid#199612PurposeEncodes DARPin-FLAG E3_5 for bacterial expression and column purificationDepositorInsertDARPin-FLAG E3_5 (control)
Tags8xHis and FLAGExpressionBacterialPromoterT5Available SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK66
Plasmid#176606PurposeTo measure Syk intensity in response to ligand clustering using DNA origamiDepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
P442
Plasmid#139728PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsiveDepositorInsertpMOD_D_pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromoterAtRAB18Available SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-Cre
Plasmid#122562PurposeIntegration of Cre sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-Cre-SV40pA
UseCloningAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLIP38
Plasmid#65616PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edCit-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3
Plasmid#122092PurposeAAV plasmid for expressing rsCaMPARI in neurons, nucleus localizedDepositorInsertrsCaMPARI
UseAAVTagsNLS-His and mRuby3Promoterhsyn (synapsin-1)Available SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
P223
Plasmid#139720PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, CPHDepositorInsertpMOD_A'_pLHB1B1:NnCPH:tSlH4
UseLuciferaseExpressionPlantPromoterAtLHB1B1Available SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA (KmR)
Plasmid#102321Purposedrives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)DepositorInsertRpaA with p0050 promoter (+ kanamycin resistance casette)
UseCloning vectorPromoter-Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP43
Plasmid#65618PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-BFLF1
Plasmid#162626PurposeExpresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cellsDepositorInsertBFLF1
TagsN-terminal 2xStrep-Tag IIExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLIP37
Plasmid#65505PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-Cer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn CCL20-pre-mGRASP
Plasmid#173117PurposeExpresses CCL20 sender construct in neuronsDepositorInsertCCL20-pre-mGRASP
UseAAVPromoterSynapsinAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Paxillin LD
Plasmid#186150PurposeFor expressing recombinant paxillin variants in bacteria.DepositorInsertPXN (PXN Human)
TagsGSTExpressionBacterialMutationD5A; D146A; D232A; D267A; D301APromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Paxillin
Plasmid#186143PurposeFor expresseion of recombinant paxillin in bacteriaDepositorInsertPXN (PXN Human)
TagsGSTExpressionBacterialMutationPaxillin C1997A (silent mutation for cloning)PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3195
Plasmid#172695PurposePunc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)DepositorInsertPunc-25
TagsGFP 1-10ExpressionWormAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::RpaA D53A
Plasmid#105229Purposeinducible expression of unphosphorylatable variant of RpaA (D53A)DepositorInsertrpaA driven by Ptrc promoter
UseCyanobacteria cloning vectorMutationD53 mutated to APromoterptrcAvailable SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Char-BFP2-TSERex
Plasmid#124792PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertChar-BFP2
ExpressionMammalianPromoterCAGAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseExpressionPlantPromoterAtuMASAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w
Plasmid#162481PurposeA bicistronic construct - FloxGal80 and UAS-mCD8GFPDepositorInsertCre80Tom-GFP
UseCre/LoxAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK33/intein-Cas9_S219-3XFLAG
Plasmid#133782PurposeExpresses human codon-optimized intein-Cas9DepositorInsertHuman codon-optimized intein-Cas9
ExpressionInsectAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx4
Plasmid#104474PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
TagsFKBPx4-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP154
Plasmid#106360PurposeA vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.DepositorTypeEmpty backboneExpressionWormAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LexAop2->Bxb1.STOP>myr::4xSNAPf
Plasmid#87640PurposeFor creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPfDepositorInsertmyr::4xSNAPf
ExpressionInsectAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only