We narrowed to 1,093 results for: pcs2

1. pCS2-3HA-Mefv iso1

   Plasmid

   #92105

   Purpose

   Expression in eukaryotic cells

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   Mefv isoform1 (Mefv Mouse)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pCS2-nCas9n-nanos 3’UTR

   Plasmid

   #62542

   Purpose

   expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

   Insert

   nanos 3' UTR (nanos3 Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   3' UTR only

   Promoter

   SP6

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+mScarlet-C Cloning Vector

   Plasmid

   #128147

   Purpose

   To express a protein of interest fused to the C-terminus of mScarlet

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   mScarlet fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2 mt-GFP (BamHI minus)

   Plasmid

   #15681

   Depositor

   Michael Klymkowsky

   Type

   Empty backbone

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   6x Myc and GFP

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2-mCherry-Rab11a (Q70L)

   Plasmid

   #184037

   Purpose

   Expresses constitutively active mCherry Rab11a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

   Insert

   Rab11
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Glutamine 70 changed to Leucine

   Promoter

   CMV IE94

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2 GFP-GSK3-MAPK

   Plasmid

   #29689

   Purpose

   GFP reporter protein fused to three GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site primed by MAPK
   

   Use

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site

   Promoter

   SP6

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-NXE+mem-KillerRed

   Plasmid

   #45761

   Depositor

   Michael Levin

   Insert

   Membrane-bound KillerRed
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   CMV IE94

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2+StayGold-C Cloning Vector

   Plasmid

   #208337

   Purpose

   To express a protein of interest fused to the C-terminus of StayGold in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   StayGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2+N-3xFlag Cloning Vector

   Plasmid

   #196651

   Purpose

   N-terminal 3xFlag mammalian expression plasmid

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2 GFP-GSK3mut-MAPK

    Plasmid

    #29690

    Purpose

    GFP reporter protein fused to three mutated GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    

    Use

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site, GSK3 …

    Promoter

    SP6

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+MCS-P2A-sfGFP

    Plasmid

    #74668

    Purpose

    Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.

    Depositor

    Jason Berman

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2-CIB1-mCherry-Rab8A

    Plasmid

    #194333

    Purpose

    For use in light-induced Rab8a protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Inserts

    CIB1 (CIB1 Mustard Weed)
    Rab8a (RAB8A Canis Lupus)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2-mCherry-Rab11a (S25N)

    Plasmid

    #184036

    Purpose

    Expresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

    Insert

    Rab11
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Serine 25 changed to Asparagine

    Promoter

    CMV IE94

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2 Notch1 ΔEMV-6MT

    Plasmid

    #41737

    Depositor

    Jeffrey Nye, Raphael Kopan

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Extracellular-domain-deleted construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 protein fragment lacking …

    Promoter

    Available Since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2-FLAG-hEcto/Tif1g

    Plasmid

    #20902

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

    Insert

    Ectodermin (TRIM33 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal deletion of 37 aminoacids; Ectodermin …

    Promoter

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCS2 Notch1 ICv-6MT

    Plasmid

    #41730

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Intracellular Domain (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 protein beginning at Vali…

    Promoter

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2plus-mVenus1-155-GGGS

    Plasmid

    #162610

    Purpose

    Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains aa1-155 of mVenus

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ZE32 Prickle-1-GFP-pCS2+

    Plasmid

    #17073

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    Prickle-1 (prickle1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCS2-CIB1-mCherry-Rab11

    Plasmid

    #140573

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Inserts

    CIB1 (CIB1 Mustard Weed)
    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2+-Y276H PHYB-CAAX

    Plasmid

    #154911

    Purpose

    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

    Depositor

    Clare Buckley, Jonathan Clarke

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge