We narrowed to 11,977 results for: pcDNA

1. pcDNA5-FRT/TO-Flag-RNF169

   Plasmid

   #74243

   Purpose

   Expresses RNF169 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

   Insert

   RNF169 (RNF169 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

   Plasmid

   #112060

   Purpose

   Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2
   

   Use

   Tags

   Nuclear Export Signal (NES) derived from HIV-1

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Promoter

   CMV

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hBACE1(D289N).ires.mCherry-pcDNA3

   Plasmid

   #204363

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   BACE1 (BACE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D289N

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Tsapphire-StagRFP-SrcAR

   Plasmid

   #173018

   Purpose

   Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Tsapphire-StagRFP-SrcAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA6-ARID1A 1759-2285

   Plasmid

   #39476

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   expresses ARID1A aa1759-2285

   Promoter

   Available since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1(-) MID51 4xMyc Hisx6

   Plasmid

   #44598

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   MiD51 (Mief1 Mouse)
   

   Use

   Tags

   4xMyc and 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human NCKX6-FlagTag (pcDNA3.1+)

   Plasmid

   #75217

   Purpose

   Mammalian expression of SLC8B1

   Depositor

   Jonathan Lytton

   Article

   Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

   Insert

   SLC8B1 (SLC8B1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_3xFlagEzh2 RNA binding mutant

   Plasmid

   #173718

   Purpose

   RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 RNA-binding mutant
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-N-Myc_535-775 USP7

   Plasmid

   #131247

   Purpose

   Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-N-Myc_775-1102 USP7

    Plasmid

    #131250

    Purpose

    Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/Puro-CAG-ASAP2s

    Plasmid

    #101274

    Purpose

    Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO HIS DNAJB6a

    Plasmid

    #19556

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6a (DNAJB6 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1(-) mouse Egr3

    Plasmid

    #107998

    Purpose

    Expresses mouse Egr3 in mammalian cells

    Depositor

    Peter Johnson

    Article

    Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

    Insert

    Egr3 (Egr3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2/V5 hsa-mir-150

    Plasmid

    #26310

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-150 (MIR150 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Casp7 C186A-Flag

    Plasmid

    #11816

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 7 catalytic mutant (CASP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C186A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 EWS-myc-HIS

    Plasmid

    #46386

    Purpose

    expresses myc, HIS tagged EWSR1 in mammalian cells

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5/FRT/TO-DDX54-FLAG/HA

    Plasmid

    #97062

    Purpose

    Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells

    Depositor

    Markus Landthaler

    Article

    Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

    Insert

    DDX54 (DDX54 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT/TO-FLAG-SHLD2

    Plasmid

    #114117

    Purpose

    Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3.1(-)-EGFP-FP4-mito

    Plasmid

    #229692

    Purpose

    Transient expression of EGFP FP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Mitochondira FP4 motifs from Listeria ActA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(-)-EGFP-AP4-mito

    Plasmid

    #229693

    Purpose

    Transient expression of EGFP AP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Control construct with tandem AP4 motifs (mitochondria)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge