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Showing: 10951 - 11000 of 169384 results

  8. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322ā€¦
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  20. AAV.rTH.PI.Cre.SV40

    Plasmid
    #107788
    Purpose
    AAV vector expressing Cre from rTH promoter
    Depositor
    James M. Wilson
    Has Service
    AAV Retrograde and AAV9
    Insert
    Cre
    Use
    AAV
    Expression
    Mammalian
    Promoter
    rTH
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    107788-plasmid-map-sequence-id-259405

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  22. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

    Plasmid
    #69100
    Purpose
    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP
    Depositor
    David Botstein
    Inserts
    yZ3EV cassette
    Z3 promoter
    yEGFP
    Expression
    Yeast
    Available Since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69100-plasmid-map-sequence-id-122356

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  31. TFORF1688

    Plasmid
    #144371
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF1A (HNF1A Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144371-plasmid-map-sequence-id-353160

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  32. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67Rā€¦
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (cā€¦
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  40. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid
    #50458
    Purpose
    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde and AAV8
    Insert
    rM3D-mCherry
    Use
    AAV
    Tags
    HA and mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50458-plasmid-map-sequence-id-162264

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Showing: 10951 - 11000 of 169384 results