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LentiU6-hASCL1 gRNA_1-MS2-Puro

Plasmid

#192670

Purpose

Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNA #1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (277-439)

Plasmid

#203435

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTYB11-PTBP1-RRM1

Plasmid

#89153

Purpose

Recombinant expression of PTBP1-RRM1 in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pVL1393-MAL-SYK

Plasmid

#157990

Purpose

Expresses SYK in insect cell (sf9)

Depositor

Byung Il Lee

Article

Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

Insert

Spleen tyrosine kinase (SYK Human)

Tags

Met-Ala-Leu and add Leu,Glu residues +His tag

Expression

Insect

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR-27a-3p

Plasmid

#82438

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-27a-3p (MIR27A Human)

Mutation

Three copies hsa-miR-27a-3p Complementary region …

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-C1-FKBP-CYB5tail

Plasmid

#202686

Purpose

rapamycin-induced recruitment of proteins to ER

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB9

Plasmid

#19532

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K9-2xCSTREP

Plasmid

#136160

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K9

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-PPxY

Plasmid

#61006

Purpose

Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 PPxY mutation (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

PPxY mutation

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
GW1-mito-mRuby2-roGFP1

Plasmid

#107219

Purpose

Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

mRuby2-roGFP1

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-DNMT3a ts1

Plasmid

#115865

Purpose

DNMT3a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

DNMT3a shRNA (DNMT3A Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-TEV-HsRCK_296-472_E

Plasmid

#146209

Purpose

Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsRck_296-472 (DDX6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (71-181)

Plasmid

#86753

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 71-181

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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GABPA_pet28a

Plasmid

#131640

Purpose

Bacterial expression of ETS gene GABPA

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

GABPA (GABPA Human)

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF74-2xNSTREP

Plasmid

#136233

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF74

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF74

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CSK-1/1

Plasmid

#91542

Purpose

Protein expression and purification of human SH3 domain construct CSK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CSK-1/1 (CSK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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QSOX1-luciferase

Plasmid

#113352

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

QSOX1-enhancer (QSOX1 Human)

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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anxA10-GFP

Plasmid

#107199

Purpose

Expresses human annexin A10-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

Insert

annexin A10 (ANXA10 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (1-181)

Plasmid

#86752

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-181

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Fyn-SH3_5WT_S25C

Plasmid

#118824

Purpose

Bacterial expression of the SH3 domain of human Fyn

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

FYN (FYN Human)

Tags

6-His-TEV tag

Expression

Bacterial

Mutation

S25C; See depositor comments below.

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-DNMT3a ts2

Plasmid

#115866

Purpose

DNMT3a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

DNMT3a shRNA (DNMT3A Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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ESR1-Tol2

Plasmid

#113382

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Tol2 reporter

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF62-2xCSTREP

Plasmid

#136222

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF62

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF62

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF6-2xCSTREP

Plasmid

#136170

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF6

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF6

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_266-341

Plasmid

#118821

Purpose

Bacterial expression of hnRNPA2 truncation

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His and TEV

Expression

Bacterial

Mutation

contains amino acids 266-341

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(84Q)

Plasmid

#21754

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 84 Glutamines and no…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pDK637

Plasmid

#134203

Purpose

bacterial expression of human CENP-N 1-240

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N 1-240-His (CENPN Human)

Expression

Bacterial

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC25

Plasmid

#62328

Purpose

sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x MS2 binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-P834A

Plasmid

#188082

Purpose

Expresses human ATG9-P834A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

P834A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP9A-Tol2

Plasmid

#113385

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ATP9A-enhancer (ATP9A Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Tags

6*His-mEos3.2

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 βarr1 S412A Flag

Plasmid

#42195

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412A (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412A

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO hChR2(E123A)-mCherry

Plasmid

#35508

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR221-GLI1

Plasmid

#79497

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

GLI1 (GLI1 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAMAHISTEV_PfeAQ482A

Plasmid

#128945

Purpose

expresses Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4.TO-ORF68-2xCSTREP

Plasmid

#136229

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF68

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF68

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP9A-luciferase

Plasmid

#113354

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ATP9A-enhancer (ATP9A Human)

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-V5-PTPN14-del-N

Plasmid

#61004

Purpose

Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete N (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 17-310

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneoLuc-PP2A

Plasmid

#128349

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP2A (PTPA Human)

Tags

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-Atg14 (1-200)

Plasmid

#86750

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-200

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

Plasmid

#141236

Purpose

AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox; Cre-off

Tags

6xHIS tag and nuclear export signal

Promoter

human elongation factor-1 alpha (EF-1 alpha)

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-LplA(AAG)

Plasmid

#61307

Purpose

Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

6xHis, Flag, T7 tag, and Xpress epitope

Expression

Mammalian

Mutation

E20A, F147A, H149G

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4.TO-ORF61-2xCSTREP

Plasmid

#136221

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF61

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF61

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4.TO-K1-2xCSTREP

Plasmid

#136148

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-V5-PTPN14-del-C

Plasmid

#61005

Purpose

Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete C (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 934-1187

Promoter

CMV

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Myc-huMEKK3 (K391A)

Plasmid

#44157

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

MEKK3 (K391A) (MAP3K3 Human)

Tags

Myc

Expression

Mammalian

Mutation

K391A, kinase-dead mutant

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.3−3xFLAG−AGO2(S387D)

Plasmid

#220463

Purpose

Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to aspartate

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-HA-HA-human CMYC (208-439)

Plasmid

#203433

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 19,198 results for: comp