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p6642 MSCV-P C-FlagHA 6bE7-Kozak

Plasmid

#35020

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV6b E7 (E7 HPV 6b)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
39 p ephrin B2

Plasmid

#13063

Depositor

Nathan Lawson, Michael Tsang

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

ephrin B2

Available Since

July 23, 2010

Availability

Academic Institutions and Nonprofits only
p-miR-Report + WT miR-181 BS in GATA6 3UTR

Plasmid

#22794

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 hMOG-beta1-EGFP

Plasmid

#160978

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMD1_sgRNA_5

Plasmid

#74181

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLCKO_Luciferase_sgRNA

Plasmid

#74190

Purpose

lentiviral vector expressing sgRNA targeting Luciferase

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Luciferase sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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Myc-RILPL1

Plasmid

#201470

Purpose

For overexpression in cultured cells to visualize RILPL1 protein interactions.

Depositor

Suzanne Pfeffer

Article

Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

Insert

RILPL1 (RILPL1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR wt (Shank3)

Plasmid

#175254

Purpose

SPN-ARR fragment in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment from SHANK3

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-G185R

Plasmid

#125849

Purpose

express mutant BAP1-G185R

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

G185R

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ULK2-K39I

Plasmid

#97076

Purpose

Expresses mutant ULK2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

ULK2 (ULK2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 39 to Isoleucine

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Promoter

U6

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-FLAG-UL36USP (C65)

Plasmid

#235549

Purpose

To express GFP-FLAG-UL36USP (C65) - region 1-520 of UL36

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

UL36USP (C65)

Use

Lentiviral

Tags

EGFP-FLAG

Mutation

N-terminal part of UL36 starting at the first ATG…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B

Plasmid

#203695

Purpose

expresses ATP10B

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pKTop::tse5-CT (1169-1281)

Plasmid

#192955

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1281)

Expression

Bacterial

Mutation

encodes for tse5 (1169-1281)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

Plasmid

#198753

Purpose

Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

See depositor comments below

Promoter

EF1a

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAF0034 Ef1a-Kp03-2a-EGFP

Plasmid

#193461

Purpose

Expression of Kp03 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE212.pa

Plasmid

#134930

Purpose

GEF-H1 biosensor that does not have GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CAG::Cytbow

Plasmid

#158995

Purpose

Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Cytbow

Tags

The default expressed EBFP2 is fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ Myc2-mouse TTP WT

Plasmid

#107000

Purpose

Expresses epitope-tagged wild-type murine TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (Zfp36 Mouse)

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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