We narrowed to 14,624 results for: RING
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Plasmid#199589PurposeContains guide RNA to 3' end of mouse EZH2 geneDepositorAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only
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pRSFDuetP-HsEDC3_192-228-GST-HsRCK_E
Plasmid#146211PurposeBacterial Expression of HsEDC3_192-228-HsRckDepositorInsertHsEDC3_192-228-HsRck (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E
Plasmid#146212PurposeBacterial Expression of HsEDC3_192-228-HsRck-E236QDepositorInsertHsEDC3_192-228-HsRck-E236Q (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E
Plasmid#146213PurposeBacterial Expression of HsEDC3_192-228-HsRck-K135ADepositorInsertHsEDC3_192-228-HsRck-K135A (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E
Plasmid#146215PurposeBacterial Expression of HsEDC3_192-508-HsRck-E236QDepositorInsertHsEDC3_192-508-HsRck-E236Q (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E
Plasmid#146216PurposeBacterial Expression of HsEDC3_192-508-HsRck-K135ADepositorInsertHsEDC3_192-508-HsRck-K135A (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E
Plasmid#146204PurposeInsect Expression of DmMe31B-Q281AH284ADepositorInsertDmMe31B-Q281AH284A (me31B Fly)
ExpressionInsectMutationone silent mutation A1101G and two non silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E
Plasmid#146205PurposeInsect Expression of DmMe31B-T288AK292ADepositorInsertDmMe31B-T288AK292A (me31B Fly)
ExpressionInsectMutationone silent mutation A1101G and two non silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle.CC9
Plasmid#192931PurposeGateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorPromoterCMVAvailable SinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)
Plasmid#186703PurposeMammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)DepositorInsertmiRFP703-eDHFR(69K6)-Tiam1(DH-PH)
ExpressionMammalianAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pv6_CBX7_Chromo-W35A_TAF3_Phd
Plasmid#179401PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_PhdDepositorInsertCBX7_Chromo(W35A) - TAF3_Phd
TagsAviTag and EGFPExpressionMammalianMutationChromodomain W35APromoterCAGGSAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-Flag-Mcm2 targeting sgRNA
Plasmid#186937PurposeGenomic targeting of Flag tag at Mcm2 N-terminalDepositorAvailable SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-Mcm2-2A mutation sgRNA
Plasmid#186936PurposesgRNA used for genetic mutation at Y81 and Y90 of Mcm2DepositorInsertMcm2-2A mutation sgRNA (Mcm2 Mouse)
UseCRISPR and Mouse TargetingExpressionMammalianPromoterU6Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-742pUC19-tNGFR-P2A-Caspase8
Plasmid#186099PurposeKnockin of truncated NGFR to target geneDepositorInsertCASPASE8
UseCRISPRMutationWT with tNGFR sequenceAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-MYC.Mm.EPC1
Plasmid#184653PurposeRetroviral expression of mouse Epc1DepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-MYC.Mm.EPC2
Plasmid#184654PurposeRetroviral expression of mouse Epc2DepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dendra-Rab11a
Plasmid#184038PurposeExpresses red to green photoconvertible Rab11aDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBICI_high ori
Plasmid#179273PurposeExpresses CuCBP and BaSP in E. coli BL21(DE3)DepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUBI
Plasmid#179274PurposeExpresses CcCDP monocistronically and CuCBP and BaSP bicistronicallyDepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationsucrose phosphorylasePromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo
Plasmid#179440PurposeExpresses rTetR-HDAC4(human) for gene silencing, integration by PiggybacDepositorInsertH2B-mIFP-T2A-rTetR-HDAC4
ExpressionMammalianPromoterpGKAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK_E
Plasmid#146214PurposeBacterial Expression of HsEDC3_192-508-HsRckDepositorInsertHsEDC3_192-508-HsRck (EDC3 Human)
ExpressionBacterialAvailable SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2601
Plasmid#175085PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationS47A, S65A, T75AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.4.hSyn.flex.H2B.RFP
Plasmid#170386PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.3.hSyn.flex.H2B.RFP
Plasmid#170374PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.13.hSyn.flex.H2B.RFP
Plasmid#170376PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-dVHH[GFP] in pCSDEST
Plasmid#162870PurposeSuper-resolution of GFP in cultureDepositorInsertmEos2-dVHH[EGFP]
TagsmEos2ExpressionMammalianAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA
Plasmid#173213PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC002
Plasmid#171139PurposeFor integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertsJ1-BBa_J23117-sfGFP
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterJ1-BBa_J23117 and Sp.pCas9, BBa_J23107Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-MCS-EnR_C-term
Plasmid#136885PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codonDepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaLoop-MIDAS
Plasmid#171605PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
TagsHis6 tag and SNAP tagExpressionBacterialMutationReplaced amino acids 4458-4496 with ASGSGS linkerAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM L368G
Plasmid#169985PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutationsDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM R394E
Plasmid#169987PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutationsDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM F405G
Plasmid#169988PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutationsDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM F370G
Plasmid#169986PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND F405G + S297A
Plasmid#169974PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND E274R + S297A
Plasmid#169968PurposepDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E + S297A
Plasmid#169972PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM E274R
Plasmid#169977PurposepDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A
Plasmid#169955PurposeMammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A
Plasmid#169957PurposeMammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A
Plasmid#169951PurposeMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/R
Plasmid#166976PurposeExpresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 AQ/Y
Plasmid#166975PurposeExpresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-As
Plasmid#138097PurposeGolden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only