Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 23,150 results for: CRISPR

Showing: 4401 - 4420 of 23150 results

  1. pENTRY4_ Dja6_1

    Plasmid
    #126894
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126894-plasmid-map-sequence-id-249699

    Enlarge

  2. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid
    #192664
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB-MeCP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192664-plasmid-map-sequence-id-379868

    Enlarge

  3. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

    Enlarge

  4. plenti guide Thy1.1

    Plasmid
    #128063
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128063-plasmid-map-sequence-id-255154

    Enlarge

  5. pX551-CMV-SpCas9

    Plasmid
    #107024
    Purpose
    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107024-plasmid-map-sequence-id-208524

    Enlarge

  6. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161815-plasmid-map-sequence-id-320654

    Enlarge

  8. pTRE3G-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192669
    Purpose
    Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192669-plasmid-map-sequence-id-379887

    Enlarge

  9. pZFX-donor

    Plasmid
    #112366
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFX with GFP
    Depositor
    Kevin White
    Insert
    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    Use
    CRISPR
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112366-plasmid-map-sequence-id-229714

    Enlarge

  10. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

    Enlarge

  11. PX380 Treponema denticola Cas9

    Plasmid
    #68317
    Purpose
    Treponema denticola Cas9
    Depositor
    Feng Zhang
    Insert
    TdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68317-plasmid-map-sequence-id-119529

    Enlarge

  12. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

    Enlarge

  13. CMV-dCas9_puroR

    Plasmid
    #219825
    Purpose
    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    CMV
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219825-plasmid-map-sequence-id-441124

    Enlarge

  14. pB-CAG-PE2-NatMX

    Plasmid
    #158567
    Purpose
    Piggybac vector that allows for the generation of PE2-expressing stable cell lines
    Depositor
    Alejandro Chavez
    Insert
    PE2
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158567-plasmid-map-sequence-id-309882

    Enlarge

  15. pSBtet-RB-PE2

    Plasmid
    #173903
    Purpose
    SB-transposon with inducible expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173903-plasmid-map-sequence-id-346343

    Enlarge

  16. pZBTB5-donor

    Plasmid
    #112303
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
    Use
    CRISPR
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112303-plasmid-map-sequence-id-228415

    Enlarge

  17. pRDB_031

    Plasmid
    #245331
    Purpose
    Cas12a CRISPRko all-in-one positive control; targets CD47, CD63
    Depositor
    John Doench, David Root
    Insert
    Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245331-plasmid-map-sequence-id-488051

    Enlarge

  18. pRDB_245

    Plasmid
    #245332
    Purpose
    Cas12a CRISPRko positive control guide; targets CD46
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245332-plasmid-map-sequence-id-488043

    Enlarge

  19. pRDA_837

    Plasmid
    #245328
    Purpose
    Cas9 CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245328-plasmid-map-sequence-id-488040

    Enlarge

  20. pRDB_247

    Plasmid
    #245334
    Purpose
    Cas12a CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245334-plasmid-map-sequence-id-488032

    Enlarge

Showing: 4401 - 4420 of 23150 results