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pS6-hα2M-H6

Plasmid

#128495

Purpose

Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-S1468) (A2M Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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CFAP410_pcDNA6.2/EmGFP-Bsd

Plasmid

#176948

Purpose

Mammalian expression vector encoding CFAP410 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CFAP410 (C21orf2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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miniSOG-LaminB1-10

Plasmid

#57771

Purpose

Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LaminB1 (LMNB1 Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-Nsp8

Plasmid

#165114

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp8 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (91-536)

Plasmid

#42206

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (91-536) (SRC Human)

Expression

Mammalian

Mutation

contains amino acids 91-536

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
M3-PSCFP2

Plasmid

#114185

Purpose

studying clustering of M3 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

Insert

Muscarin acetylcholine receptor 3 (CHRM3 Human)

Tags

3xHA and PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Nsp13 K289A-EGFP

Plasmid

#165130

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 K289A (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp10-EGFP

Plasmid

#165115

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp10 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV superYFP-AURKA-mTurq2

Plasmid

#157772

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and superYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa:polyA

Plasmid

#193012

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa (NRG1 Human)

Use

Transposon transgenesis

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-RFP657-CASP8

Plasmid

#75164

Purpose

LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

CASP8 sgRNA (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11

Plasmid

#184277

Purpose

Expresses nanobody H11 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(+)-ATI_QconCAT_1

Plasmid

#163957

Purpose

Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

Depositor

Stefan Tenzer

Article

Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

Insert

ATI QconCAT

Tags

polyhistidine tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP3

Plasmid

#41667

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 3 C-terminal-m…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Zyxin-6

Plasmid

#57802

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Zyxin (ZYX Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pIE-hα2M-H6

Plasmid

#128492

Purpose

Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (A2M Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

Plasmid

#146392

Purpose

Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmeIF4G-trunc (eIF4G1 Fly)

Expression

Insect

Mutation

322-1666 truncated version of elF4G sequence with…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_NOPLight-ctr

Plasmid

#195579

Purpose

Expresses the control sensor NOPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

Insert

NOPLight-ctr

Expression

Mammalian

Mutation

D110A, D130A

Promoter

CMV

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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KCNE2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176980

Purpose

Mammalian expression vector encoding KCNE2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

KCNE2 (KCNE2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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