We narrowed to 7,101 results for: tev

1. SpyCatcher002-oDi

   Plasmid

   #124661

   Purpose

   Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oDi
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST14-Cys-SpyCatcher002

   Plasmid

   #102829

   Purpose

   Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Cys-SpyCatcher002
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Cysteine residue in N-terminal region

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AviTag-SpyCatcher

   Plasmid

   #72326

   Purpose

   Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   AviTag-SpyCatcher
   

   Use

   Tags

   N-term His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_human-Caspase-4_enzymatic-domain

   Plasmid

   #183381

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4 (AA94-377) (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-APOBEC1-YTH

   Plasmid

   #200158

   Purpose

   For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTH
   

   Use

   Tags

   6xHis, HA, and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-Halo-10-PFN1-Y6D

   Plasmid

   #204223

   Purpose

   Expression of Halo-Profilin-1(Y6D) in tissue culture cells

   Depositor

   Jessica Henty-Ridilla

   Article

   Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

   Insert

   PFN1 (PFN1 Human)
   

   Use

   Tags

   Halo, TEV site, SGLRSRAQAS linker

   Expression

   Mammalian

   Mutation

   Y6D

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Halo-10-PFN1-R88E

   Plasmid

   #204224

   Purpose

   Expression of Halo-Profilin-1(R88E) in tissue culture cells

   Depositor

   Jessica Henty-Ridilla

   Article

   Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

   Insert

   PFN1 (PFN1 Human)
   

   Use

   Tags

   Halo, TEV site, SGLRSRAQAS linker

   Expression

   Mammalian

   Mutation

   R88E

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyCatcher002-oTet

   Plasmid

   #124663

   Purpose

   Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oTet
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpyCatcher002-oTri

   Plasmid

   #124662

   Purpose

   Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oTri
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_mouse-pro-IL18

    Plasmid

    #183389

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (Il18 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCB168

    Plasmid

    #37389

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIC-ZB

    Plasmid

    #26107

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    His6 - Z-basic - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL2108

    Plasmid

    #201969

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

    Insert

    attB-3C-eGFP-4xGly-TEV-Flag-6xHis
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ubiquitin K48C

    Plasmid

    #86588

    Purpose

    Bacterial expression of Ubiquitin K48C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K48C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pDEST14-Avitag-SpyCatcher002

    Plasmid

    #102828

    Purpose

    Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Avitag-SpyCatcher002
    

    Use

    Tags

    Avitag, GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Erk2

    Plasmid

    #29582

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    HIS6 and TEV

    Expression

    Bacterial

    Mutation

    Starting Met removed from Erk2

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpyCatcher002-oHex

    Plasmid

    #124670

    Purpose

    Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oHex
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Use

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpyCatcher002-oPent

    Plasmid

    #124664

    Purpose

    Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oPent
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge