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Showing: 4461 - 4480 of 7626 results

  2. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid
    #207769
    Purpose
    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207769-plasmid-map-sequence-id-413794

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  7. BDKBR1-DuET

    Plasmid
    #213189
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    BDKBR1 (BDKRB1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213189-plasmid-map-sequence-id-430754

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  10. pcDNA3.1-mPCSK3 H194L

    Plasmid
    #122689
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Expression
    Mammalian
    Mutation
    Changed Histidine 194 to Leucine
    Available Since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122689-plasmid-map-sequence-id-244742

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  12. pCS-CG-Lock-Cer-VWF

    Plasmid
    #124828
    Purpose
    Expresses multimeric human VWF-ECFP fusion protein with A2-Lock domain The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.
    Depositor
    Sriram Neelamegham
    Article
    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:
    Insert
    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains and Lock A2 domain (VWF Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124828-plasmid-map-sequence-id-243927

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  16. ECE1-His-bio

    Plasmid
    #53361
    Purpose
    Expresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ECE1 (ECE1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53361-plasmid-map-sequence-id-83086

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  17. LUC2 (L/Sec/deltaP)

    Plasmid
    #112145
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and d…
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112145-plasmid-map-sequence-id-214904

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Showing: 4461 - 4480 of 7626 results