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pMyc-CMV1-huTLR4mut-C1196T

Plasmid

#53526

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C1196T (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

C1196T

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1-huTLR4mut-A896G

Plasmid

#53525

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 A896G (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

A896G

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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LCV2 V-2

Plasmid

#84805

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43-YA/VD-Myc

Plasmid

#60701

Purpose

mammalian expression of Myc tagged rat Cx43 YA/VD mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

Myc

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)

Plasmid

#51390

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Mutation

miR-29 binding site mutant (position 577-599, 997…

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d344-873

Plasmid

#21732

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Available Since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d1-343

Plasmid

#21733

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the amino-terminus, amino-acids 1 to …

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pFastBac1/CALR del52-His

Plasmid

#214795

Purpose

Baculovirus expression of human CALR del52-His

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52-His (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR wtΔKDEL

Plasmid

#214791

Purpose

Mammalian expression of human CALR wtΔKDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wtΔKDEL (CALR Human)

Use

Retroviral

Tags

non

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wtΔKDEL-FLAG-KDEL

Plasmid

#214792

Purpose

Mammalian expression of human CALR wtΔKDEL-FLAG-KDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALRwtΔKDEL-FLAG-KDEL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mHif1a-T163A

Plasmid

#242810

Purpose

Lentiviral vector expressing mouse Hif1a-T163A mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T163A

Promoter

Ubiquitin C

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1-Aoah

Plasmid

#233141

Purpose

Thy1.1 and Aoah overexpression

Depositor

Peng Jiang

Article

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Insert

Acyloxyacyl hydrolase (Aoah Mouse)

Use

Lentiviral

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mHif1a-T163E

Plasmid

#242811

Purpose

Lentiviral vector expressing mouse Hif1a-T163E mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T163E

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-StayGold

Plasmid

#213790

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-StayGold fusion protein

Depositor

Hajime Hirase

Article

Knak et al Neurophotonics, Vol. 12, Issue S2, S2281

Insert

Alb (Alb Mouse)

Use

AAV

Tags

StayGold

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-p120RasGAP-714-1047-WT

Plasmid

#245307

Purpose

Expresses GAP domain of human p120RasGAP (residues 714-1047 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

RASA1 (RASA1 Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 1-713

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET-p120RasGAP-581-1047-WT

Plasmid

#246487

Purpose

Expresses C2 and GAP domains of human p120RasGAP (residues 581-1047 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

RASA1 (RASA1 Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 1-580

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLVXNeo_SPARC-mSc-SBP

Plasmid

#234885

Purpose

Expresses mScarlet-i and SBP tagged human SPARC for protein visualisation and use in the RUSH trafficking system

Depositor

Nicola Stevenson

Article

Thompson et al J Cell Biol. 2025 Oct 6;224(10):e2024111

Insert

SPARC (SPARC Human)

Use

Lentiviral

Tags

SBP and mScarleti

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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L4275 hIgKLSS_hTNF in pcDNA backbone

Plasmid

#244182

Purpose

Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TNF with IgK light chain secretion sequence (TNF Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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