174,600 results

Showing: 4521 - 4540 of 174600 results

Results per page:

Sort results by:

pGWB-nLUC

Plasmid

#174050

Purpose

Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay

Depositor

Alberto Macho

Article

Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

Type

Empty backbone

Use

Luciferase

Tags

N-terminal luciferase

Expression

Plant

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS008: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163045

Purpose

GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

Plasmid

#160141

Purpose

Cre- dependent expression of Sun1GFP for nuclei isolation

Depositor

Andreas Pfenning

Article

Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

Insert

Sun1-GFP (Sun1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

N-truncated: removed amino acids 1-207, retained …

Promoter

Ef1a

Available Since

March 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1/PD-L1

Plasmid

#121478

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CARPID BASU-dCasRx

Plasmid

#153209

Purpose

express CARPID BASU-dCasRx fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Article

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

BASU-dCasRx

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro

Plasmid

#141381

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp15 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

Plasmid

#135955

Purpose

Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) N-terminal

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mRuby3-C1

Plasmid

#127808

Purpose

mRuby3 C1 Cloning vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mRuby3

Expression

Mammalian

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pm-iATPSnFR1.1

Plasmid

#102549

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.1

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-AAVS1

Plasmid

#113194

Purpose

Encodes gRNA for human AAVS1

Depositor

Adam Karpf

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-SUMO-hGSDMD

Plasmid

#111559

Purpose

Express human GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (GSDMD Human)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-EF1a-TetOn3G

Plasmid

#104543

Purpose

PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

Tet-On 3G

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-mScarlet-I

Plasmid

#104007

Purpose

Plasmid encoding mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet-I

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP1174

Plasmid

#104162

Purpose

Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge