59,541 results

1. pLC242-GFP-metap2

   Plasmid

   #100518

   Purpose

   Expresses GFP tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

   Plasmid

   #220090

   Purpose

   Recruitable PIP5K to make PIP2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PIP5K1C (PIP5K1C Human)
   

   Tags

   TagBFP-FKBP1A(3-108)

   Expression

   Mammalian

   Mutation

   amino acids 1-635; D101R, R304D, R445E, K446E

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-Wnt7A

   Plasmid

   #35914

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt7A (WNT7A Human)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQCXIP mScarlet-MOSPD2 WT

   Plasmid

   #186472

   Purpose

   Expression of human MOSPD2 fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TGFB1-bio-His

   Plasmid

   #52185

   Purpose

   Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TGFB1 (TGFB1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-F-AF9

   Plasmid

   #49428

   Purpose

   Mammalian expression of Flag-tagged human AF9

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   AF-9 (MLLT3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. STK11 gRNA (BRDN0001146194)

   Plasmid

   #75913

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK11

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK11 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF3167

   Plasmid

   #144643

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF512B (ZNF512B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-2strep-FBH1

   Plasmid

   #236464

   Purpose

   transient overexpression of FBH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Jeong et al J Cell Biol. 2013 Jan 21;200(2):141-9. d

   Insert

   FBH1 (FBXO18 Human)
   

   Expression

   Mammalian

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pF146 pSOD1WTAcGFP1

    Plasmid

    #26407

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AFDN_PDZ_1

    Plasmid

    #103938

    Purpose

    Protein expression and purification of TRITHORAX PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    AFDN (AFDN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains AA983-1110 of AFDN

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG_PCP-T5Exo

    Plasmid

    #214736

    Purpose

    encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_PCP-T5Exo_bGH
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-Rab8a-wt

    Plasmid

    #86075

    Purpose

    A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human Parkin (1-465)

    Plasmid

    #110758

    Purpose

    Bacterial expression of human Parkin

    Depositor

    David Komander

    Article

    Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

    Insert

    PRKN (PRKN Human)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-Bak

    Plasmid

    #32564

    Depositor

    Richard Youle

    Article

    Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

    Insert

    human Bak (BAK1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CL20_mEGFP_KIAA1549_BRAF

    Plasmid

    #205838

    Purpose

    Express mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    KIAA1549_BRAF (KIAA1549, BRAF Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid

    #227322

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. G6PD/pRK5

    Plasmid

    #41521

    Depositor

    Xiaolu Yang

    Article

    Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

    Insert

    glucose-6-phosphate dehydrogenase (G6PD Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCW57.1 Lysosomal-METRIQ

    Plasmid

    #135401

    Purpose

    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    DNAse II alpha (DNASE2 Human)
    

    Use

    Lentiviral

    Tags

    T2A, mCherry, and sfGFP

    Expression

    Mammalian

    Mutation

    Single-nucleotide polymorphism C432T

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge