We narrowed to 15,826 results for: grna

1. pYPQ141-ZmUBI-RZ-Cas12j2

   Plasmid

   #189781

   Purpose

   Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   gRNA cloning site
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPN459

   Plasmid

   #137875

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAZ-SK

   Plasmid

   #73962

   Purpose

   self-killing gRNA plasmid pMAZ-SK

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNR4A1.1.0-gDNA

   Plasmid

   #112391

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

   Depositor

   Kevin White

   Insert

   NR4A1 (NR4A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTEAD1.1.0-gDNA

   Plasmid

   #112425

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

   Depositor

   Kevin White

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTFAP2B.1.0-gDNA

   Plasmid

   #113791

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2B

   Depositor

   Kevin White

   Insert

   TFAP2B (TFAP2B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZNF423.1.0-gDNA

   Plasmid

   #113790

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF423

   Depositor

   Kevin White

   Insert

   ZNF423 (ZNF423 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREST.1.0-gDNA

   Plasmid

   #113774

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor REST

   Depositor

   Kevin White

   Insert

   REST (REST Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-U6-MYC-Guide-hSpCas9

   Plasmid

   #228574

   Purpose

   Expression of human MYC gRNA; CRISPR mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   MYC gRNA (MYC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid

    #214688

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Puromycin resistance cassette has silent mutation…

    Promoter

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDual_dsCas9_Puro_hs_TP73

    Plasmid

    #214689

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Puromycin resistance cassette has silent mutation…

    Promoter

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZNF462.1.0-gDNA

    Plasmid

    #112476

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462

    Depositor

    Kevin White

    Insert

    ZNF462 (ZNF462 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTBPL1.1.0-gDNA

    Plasmid

    #112412

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1

    Depositor

    Kevin White

    Insert

    TBPL1 (TBPL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMAD3.1.0-gDNA

    Plasmid

    #112407

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3

    Depositor

    Kevin White

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDECKO_TFRC_C (with mCherry)

    Plasmid

    #72627

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO_UCA1

    Plasmid

    #72628

    Purpose

    Expresses two gRNAs targeting the UCA1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward UCA1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDECKO_Malat1_A

    Plasmid

    #72620

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDECKO_Malat1_C

    Plasmid

    #72622

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTC364

    Plasmid

    #91213

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6T7G

    Plasmid

    #71463

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-sgCdkn2a/Cre

    Plasmid

    #89644

    Purpose

    Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Cdkn2a (Cdkn2a Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-sgKeap1/Cre

    Plasmid

    #89645

    Purpose

    Expresses a Keap1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Keap1 (Keap1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDECKO_TFRC_B

    Plasmid

    #72626

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RFN_EGFP_81

    Plasmid

    #60073

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pDECKO_Malat1_B

    Plasmid

    #72621

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge