We narrowed to 5,570 results for: SPI

1. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

   Plasmid

   #171594

   Purpose

   Strong human ACE2 abundance from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   ACE2 (ACE2 Human)
   mCherry
   H2A
   Pac
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ApoE3

   Plasmid

   #197147

   Purpose

   Expresses ApoE3 in Mammalian cell

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   ApoE3 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC1-hVAP-A

   Plasmid

   #104447

   Purpose

   Expression of human VAP-A fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-CMV-dnMCAK

   Plasmid

   #205993

   Purpose

   Over-expression of GFP-dnMCAK

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   dnMCAK
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-UbC

   Plasmid

   #62806

   Purpose

   To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

   Depositor

   Pantelis Tsoulfas, Jae Lee

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Type

   Empty backbone

   Use

   AAV; Eucaryotic

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pg-HIF-1alpha-EGFP

   Plasmid

   #87204

   Purpose

   C-terminally EGFP-tagged human HIF-1a

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-1a (HIF1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L40F, M88V, Q814R

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-IRES_Tomato

   Plasmid

   #107229

   Purpose

   Tomato expressing plasmid

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS2144

   Plasmid

   #104357

   Purpose

   AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

   Insert

   ssAAV-smCBA-EmGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   smCBA

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

   Plasmid

   #174515

   Purpose

   Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Inserts

   Mm PylS
   MmtRNA-Pyl-opt(UGA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ASL from the serT gene (UGA anticodon) and recoded

   Promoter

   GlnS and lpp

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETcon_SARS-CoV-2_RBD

    Plasmid

    #166782

    Purpose

    Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

    Depositor

    Jesse Bloom

    Article

    Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

    Insert

    Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV CD68-hM4D(Gi)-mCherry

    Plasmid

    #75033

    Purpose

    Expression of hM4D(Gi) using the CD68 promoter.

    Depositor

    Bryan Roth

    Article

    Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Has Service

    AAV1 and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CD68

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1 hSlp2-a

    Plasmid

    #40047

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a (SYTL2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-dnMCAK

    Plasmid

    #205995

    Purpose

    inducible expression of HA-dnMCAK

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    dnMCAK
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

    Plasmid

    #89793

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Q147L, CAA-->CTA

    Promoter

    hUbC

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pg-ODD-EGFP

    Plasmid

    #87211

    Purpose

    HIF-1a's ODD domain fused to EGFP

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-1a ODD domain (HIF1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ODD domain (AA 530-652)

    Promoter

    CMV

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-C1 hSlp2-a

    Plasmid

    #40056

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a (SYTL2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX304-MARK3

    Plasmid

    #107235

    Purpose

    Lentiviral gene expression vector for phospho-MARK3 expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc-C Med12His

    Plasmid

    #49240

    Purpose

    expresses human Med12 with His tag in insect cells

    Depositor

    Dylan Taatjes

    Article

    Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

    Insert

    Med12 (MED12 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. WIW_Scaffold2

    Plasmid

    #205574

    Purpose

    Nanobody that inhibits seeding of tau fibrils

    Depositor

    David Eisenberg

    Article

    Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    WIW_Scaffold2
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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