We narrowed to 1,876 results for: IRES-GFP

1. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102887

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

   Plasmid

   #153071

   Purpose

   Lentiviral vector expressing human ZC3H12D mutated (D95N)

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   Mutated ZC3H12D (D95N) (ZC3H12D Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed D95 to N, known to abrogate the RNase enz…

   Promoter

   EF1α

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102888

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

   Plasmid

   #134863

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAMP4 (SERP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

   Plasmid

   #200636

   Purpose

   Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

   Plasmid

   #122954

   Purpose

   Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NLB (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xhis tag

   Expression

   Mutation

   Promoter

   CAG promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-human CSFI-R-L301S-IRES-GFP

   Plasmid

   #133744

   Purpose

   retroviral expression of CSF1-R mutant

   Depositor

   Martine Roussel

   Article

   Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

   Insert

   CSF1-R (CSF1R Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L301S

   Promoter

   MSCV

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

   Plasmid

   #75115

   Purpose

   Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   deleted bromodomain

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90213

   Purpose

   To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

    Plasmid

    #75122

    Purpose

    Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    3x FLAG-tag

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65

    Plasmid

    #32569

    Depositor

    Sergey Kasparov

    Article

    Benzekhroufa et al Gene Ther. 2009 May;16(5):681-8. Epub 20

    Inserts

    EGFP
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    2TPH

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-I-Sce1-eno2:Tau-IRES-egfp

    Plasmid

    #112205

    Purpose

    Used to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoter

    Depositor

    Edward Burton

    Article

    Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

    Insert

    MAPT (MAPT Human)
    

    Use

    Zebrafish expression

    Tags

    eno2 exon 2 translational fusion, ires-egfp at c …

    Expression

    Mutation

    Promoter

    eno2 from zebrafish

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

    Plasmid

    #122991

    Purpose

    Expresses Flag-tagged hRPGRIP1L in mammalian cells

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Insert

    RPGRIP1L (RPGRIP1L Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

    Plasmid

    #164777

    Purpose

    cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

    Insert

    nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E61C

    Promoter

    pGK

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-IRES-GFP-TLR4 delta tail

    Plasmid

    #133063

    Purpose

    Retroviral expression of mouse TLR4 with out its cytosolic tail

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    TLR4 delta tail (Tlr4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-IRES-GFP-CD4-TLR4 cyto

    Plasmid

    #133064

    Purpose

    Retroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    CD4-TLR4cyto (Tlr4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

    Plasmid

    #69049

    Purpose

    lentiviral expression of IKZF2 variant 2 with FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2 (IKZF2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    CAG

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-Myc-PGK-Puro-IRES-GFP

    Plasmid

    #75124

    Purpose

    Retroviral expression plasmid encoding wild type murine Myc

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS

    Plasmid

    #131355

    Purpose

    Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    Caspase-1 (C285A)-CpLxIS (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Caspase-1 (C285A)

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

    Plasmid

    #166951

    Purpose

    Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    GFP and mCherry

    Expression

    Mutation

    SFPQ-Y527A

    Promoter

    eF1a

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

    Plasmid

    #75129

    Purpose

    Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

23. MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

    Plasmid

    #75118

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Bromodomain replaced with the bromodomain of BRD7

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

24. MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid

    #75123

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    3x FLAG-tag

    Expression

    Mutation

    bromodomain replaced with the first bromodomain o…

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pCIG-Flag-Sox9-IRES-nls-GFP

    Plasmid

    #44281

    Depositor

    Martin Cheung

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

    Insert

    Flag-Sox9 (SOX9 Chicken)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE enhancer and Chicken beta-actin promoter

    Available since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

26. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102891

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

    Plasmid

    #75126

    Purpose

    Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    KMT6 (EZH2 Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    SET domain replaced with the SET domain of EZH1

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

30. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

    Plasmid

    #63060

    Purpose

    Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    nls-mRuby2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge