We narrowed to 14,407 results for: nts

1. pYO831

   Plasmid

   #235754

   Purpose

   Protein expression of ZZ-ScVPS38 (1-212F)

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS38
   

   Tags

   ZZ-3xTEV

   Expression

   Yeast

   Mutation

   aa 1-212F

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYO225

   Plasmid

   #235729

   Purpose

   Protin expression of FL yeast VPS15 -ZZ in yeast

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS15
   

   Tags

   3xTEV-ZZ

   Expression

   Yeast

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYO290

   Plasmid

   #235731

   Purpose

   Protein expression in FL yeast VPS34 D731N mutant in yeast

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS34
   

   Expression

   Yeast

   Mutation

   S2A, D731N

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYO478

   Plasmid

   #235757

   Purpose

   Expression of FL ScVPS34-3xFLAG under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS34
   

   Tags

   3xFLAG

   Expression

   Yeast

   Promoter

   ScVPS34 promoter

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYO764

   Plasmid

   #235761

   Purpose

   Expression of ScVPS38-EGFP under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS38
   

   Tags

   EGFP

   Expression

   Yeast

   Promoter

   ScVPS38 promoter

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYO932

   Plasmid

   #235764

   Purpose

   Expression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS34 HELCAT
   

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   aa 268-875

   Promoter

   ScVPS34 promoter

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNTI763 p(Z)-mCherry + UBC9 sgRNA

   Plasmid

   #164918

   Purpose

   CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   P(Z)-mcherry
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   P(Z), synthetic promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

   Plasmid

   #164916

   Purpose

   Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   GCN4(UTR)-ZEM(TF)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   PGK1 (yeast)

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

   Plasmid

   #115432

   Purpose

   Vector for expressing RPL28 with mutagenized exon2

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Expression

   Yeast

   Mutation

   exon 2 deleted, with MfeI / NheI cloning site

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNP2390 [pNP2322 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

    Plasmid

    #247252

    Purpose

    Measure ISCro4 activity via mCherry expression

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2322 - ISCro4_Donor, ISCro4_Target_RTG-Matched
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP1954

    Plasmid

    #222730

    Purpose

    pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH41331::pU6cm-pegR::EURb7

    Plasmid

    #227695

    Purpose

    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6 composite

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH47742::p35S-PE2max-NC::tEURb7

    Plasmid

    #227693

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pRPS5a

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNTI935

    Plasmid

    #216638

    Purpose

    cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID-PM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNP568

    Plasmid

    #222725

    Purpose

    pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNP1723

    Plasmid

    #222727

    Purpose

    sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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