We narrowed to 5,331 results for: pcdna3.1

1. pcDNA3.1-E-Flag

   Plasmid

   #101858

   Purpose

   Evi-1 flag tagged for transfection into mammalian cells

   Depositor

   Archibald Perkins

   Insert

   Evi-1 (Mecom Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 TREK-2

   Plasmid

   #224769

   Purpose

   In vitro transcription for Oocyte expression of 1 TREK-2

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily member 10 (Kcnk10 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Promoter

   T7

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-LGC-53

   Plasmid

   #86399

   Purpose

   Mammalian expression of codon-optimized dopamine receptor LGC-53

   Depositor

   Steven Mennerick

   Article

   Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

   Insert

   LGC-53 (lgc-53 Synthetic, Nematode)
   

   Expression

   Mammalian

   Mutation

   Codon optimized for mammalian expression; Signal …

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 GW-NL-myc

   Plasmid

   #113447

   Purpose

   NanoLuc Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Luciferase; Gateway shuttle vector

   Tags

   NanoLuc-cmyc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1Xpress-BcrGAP

   Plasmid

   #38175

   Depositor

   Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   BCR (BCR Human)
   

   Tags

   Xpress

   Expression

   Mammalian

   Mutation

   contains GAP domain amino acids 1004-1271

   Available Since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1(+) M-Ras(WT)

   Plasmid

   #224287

   Purpose

   Cell transfection and expression of M-Ras

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   M-Ras (MRAS Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(-)_mtZFN(-)2G

   Plasmid

   #62799

   Purpose

   mtZFN2G architecture bearing (-) FokI domain

   Depositor

   Michal Minczuk

   Article

   Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

   Insert

   mtZFN(-)2G
   

   Tags

   Internal FLAG tag immediately after F1B mitochond…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(-)_mtZFN(+)2G

   Plasmid

   #62798

   Purpose

   mtZFN2G architecture bearing (+) FokI domain

   Depositor

   Michal Minczuk

   Article

   Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

   Insert

   mtZFN(+)2G
   

   Tags

   Internal HA tag immediately after F1B mitochondri…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1Xpress-AbrGAP

   Plasmid

   #38177

   Depositor

   Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   Abr GAP domain (ABR Human)
   

   Tags

   Xpress

   Expression

   Mammalian

   Mutation

   ABR GAP domain including amino acids 597-859

   Available Since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1/Hygro(-)-B2-cT

    Plasmid

    #104355

    Purpose

    Expresses human beta 2 integrin tension sensor

    Depositor

    Pontus Nordenfelt, Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    Human integrin b2 with tension sensor (ITGB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_RapACR_T111C_N248Q-EYFP

    Plasmid

    #119197

    Purpose

    Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q

    Depositor

    John Spudich

    Insert

    Anion channelrhodopsin RapACR fast mutant T111C_N248Q
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed threonine 111 to cysteine and asparagine …

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 GW-mCit-PA

    Plasmid

    #113449

    Purpose

    ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Gateway shuttle vector

    Tags

    mCitrine-ProteinA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. YFP B1 in pcDNA3.1

    Plasmid

    #36397

    Depositor

    Narasimhan Gautam

    Article

    Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

    Insert

    G protein B1 (GNB1 Bovine)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    April 4, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3.1+ HA-hAO7

    Plasmid

    #137840

    Purpose

    N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 (RNF25 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-ER-BSCaM2

    Plasmid

    #64855

    Purpose

    Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-BSCaM2
    

    Tags

    CFP, N-terminal 27 amino acids from CYP450, and Y…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 PA-mCit-GW

    Plasmid

    #113448

    Purpose

    ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Gateway shuttle vector

    Tags

    ProteinA-mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-PM-CaNARi

    Plasmid

    #64850

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNARi
    

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1-EPSIN 1

    Plasmid

    #22225

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Tags

    Xpress

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1+N-eGFP_SAMD9L_R986C

    Plasmid

    #206176

    Purpose

    mammalian expression of SAMD9L R986C

    Depositor

    Christopher Goodnow

    Article

    Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

    Insert

    SAMD9L (SAMD9L Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R986C

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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