We narrowed to 20,218 results for: ACE;

1. kiCAP-AAV-PAL2

   Plasmid

   #196691

   Purpose

   Rep/Cap plasmid for the production of PAL2, an AAV capsid with CNS tropism in macaques.

   Depositor

   Pardis Sabeti

   Article

   Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   PTQGTVR insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST_DHN_AtCor47 (pBS0784)

   Plasmid

   #185254

   Purpose

   For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DHN_AtCor47
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZJQIBEBT003

   Plasmid

   #140053

   Purpose

   The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

   Depositor

   Jia Zhang

   Article

   Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

   Insert

   eMutS-L157C-G233C-CBM-EGFP
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   L157C, G233C

   Promoter

   T7

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEX-SaCas9-U6-sgGabrg1

   Plasmid

   #124856

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MTK3_001

   Plasmid

   #123952

   Purpose

   Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   3XNLS::CRY2PHR::p65
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

   Plasmid

   #124864

   Purpose

   Mutagenesis of Grin2b

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2b (Grin2b Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-MtPylRS (human-opti)

   Plasmid

   #164080

   Purpose

   To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtPylRS-478(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-MfBulKRS (human-opti)

   Plasmid

   #164196

   Purpose

   To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   Mf BulKRS(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Y269A;Y347F

   Promoter

   CMV

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-Squ5'3'

   Plasmid

   #20165

   Depositor

   Eric Wieschaus

   Article

   Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

   Insert

   spaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream (sqh Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pPKm-112

    Plasmid

    #90494

    Purpose

    pcDNA3 - pCMV - MTAD - PIF3, expresses Phytochrome interacting factor 3 (PIF3) and minimal transactivation domain (MTAD), under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    MTAD-PIF3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe_puro_DEST_Flag_YFP

    Plasmid

    #45278

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    YFP
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

12. MBC2-PuroIII

    Plasmid

    #153420

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET19b-hMED9

    Plasmid

    #15365

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

    Insert

    hMED9 (MED9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pBEST-p70a-UTR1-Nterm 6xHis PL-T500

    Plasmid

    #92314

    Purpose

    Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    B1 domain of protein L (PL)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MTK0_012

    Plasmid

    #123967

    Purpose

    Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hRosa26-BxBI landing pad
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY71-PT7-RiboJ-sfGFP-MGapt

    Plasmid

    #129119

    Purpose

    Expresses a fluorescent reporter for the quantification of cell-free protein expression.

    Depositor

    Matthew Lux

    Article

    Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

    Insert

    Superfolder GFP with Malachite Green aptamer
    

    Use

    Synthetic Biology

    Tags

    His6 and RiboJ Insulator

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCGN-HCF-1-N1011Δ382-450

    Plasmid

    #92322

    Purpose

    N-term of HCF-1 protein without amino acids 382-450

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 (HCFC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 2-1011 with internal deletion Δ382-450

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCGN-HCF-1-C600

    Plasmid

    #92324

    Purpose

    C-term of HCF-1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 C-term 1436-2035 (HCFC1 Human)
    

    Use

    Tags

    C-myc (EQKLISEED) and HA

    Expression

    Mammalian

    Mutation

    AA 1436-2035

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPKm- 226

    Plasmid

    #90497

    Purpose

    pcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PIF3-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JPF0522

    Plasmid

    #124013

    Purpose

    Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-FLEX-SaCas9-U6-sgGabrg3

    Plasmid

    #124858

    Purpose

    Mutagenesis of Gabrg3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg3 (Gabrg3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

    Plasmid

    #124863

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

    Plasmid

    #124872

    Purpose

    Mutagenesis of Gabrg3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg3 (Gabrg3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

    Plasmid

    #124871

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. JPF0454

    Plasmid

    #124039

    Purpose

    Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pPGK1-spCAS9-P2A-mAzamiGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MTK8b_009

    Plasmid

    #123896

    Purpose

    Encodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LA_hThal5.10-2-hg18
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MTK234_027

    Plasmid

    #123907

    Purpose

    Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA1 -hThal5.10-2_hg18
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO-Cre sgAdam10 v3

    Plasmid

    #158034

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Adam10 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. RCIscript-GoldyTALEN-ponzr1L

    Plasmid

    #42654

    Depositor

    Stephen Ekker

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    ponzr1 left pTAL scaffold
    

    Use

    Mrna transcription vector

    Tags

    Expression

    Mutation

    Promoter

    T3

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBEST-p70b-UTR1-deGFP-6xHis-T500

    Plasmid

    #92222

    Purpose

    Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70b (p70a promoter with one mutation)

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge