19,640 results

1. pcDNA HA HP1 beta

   Plasmid

   #24079

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 beta (CBX1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pDest-565

   Plasmid

   #11520

   Purpose

   Gateway destination vector for bacterial expression; His,GST tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis and GST

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-MET

   Plasmid

   #23889

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MET (MET Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCK-mScarlet3_C1

   Plasmid

   #189771

   Purpose

   Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   LCK-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

   Plasmid

   #85434

   Purpose

   Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   Pembrolizumab
   

   Use

   Lentiviral

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle

   Plasmid

   #16402

   Purpose

   Shuttle vector for use in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-SpyCatcher

   Plasmid

   #35044

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-Bla

   Plasmid

   #60526

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FLAG-MTOR-I2500F

   Plasmid

   #69014

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-I2500F (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Isoleucine 2500 to Phenylalanine

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSGKP-spe

    Plasmid

    #117234

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid

    #107738

    Purpose

    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

    Depositor

    Rylan Larsen

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre, dTomato
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSG5-ABL

    Plasmid

    #31284

    Depositor

    Nora Heisterkamp

    Article

    Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

    Insert

    V-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR'-NRSF-CITE-GFP

    Plasmid

    #21310

    Depositor

    Jay Nadeau

    Article

    Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

    Insert

    neuron restrictive silencer factor (Rest Mouse)
    

    Use

    Lentiviral

    Tags

    GFP (bicistronic with CITE/IRES)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Oct. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-A3-HA-ubiquitin

    Plasmid

    #220145

    Purpose

    Expresses A3-Ub for E3 substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    A3-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFLAG-Tetherin

    Plasmid

    #41070

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pMXs-mRFP1

    Plasmid

    #21315

    Purpose

    MoMLV-based Retroviral vector, expresses mRFP1

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    monomeric Red Fluorescence Protein 1
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB505

    Plasmid

    #74847

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50464

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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