20,049 results

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pcDNA4/TO mTRPM7

Plasmid

#45482

Depositor

Andrew Scharenberg

Article

Nadler et al Nature. 2001 May 31;411(6837):590-5.

Insert

TRPM7 (Trpm7 Mouse)

Use

Tetracycline inducible

Tags

Flag

Expression

Mammalian

Promoter

Tetracycline-controlled CMV

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
FUGW-APPSwe-T2A-mCherry

Plasmid

#190804

Purpose

Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Article

Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

Insert

APP (APP Human)

Use

Lentiviral

Mutation

Swedish (KM670/671NL)

Promoter

Ubi C

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTri

Plasmid

#124662

Purpose

Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTri

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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Flag p21 WT

Plasmid

#16240

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

Plasmid

#203350

Purpose

Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

Depositor

Christopher Grigsby, Molly Stevens

Insert

Nano Luciferase

Use

Template for t7-mediated in vitro transcription o…

Tags

Flanked by 5' and 3' UTRs from Xenopus …

Promoter

T7

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-BRD3

Plasmid

#65377

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD3 (BRD3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR4-YFP

Plasmid

#13018

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 4 (TLR4 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV Luciferase PGK-hygro

Plasmid

#18782

Depositor

Scott Lowe

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Luciferase and Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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Lentiviral mBmal1-Luc

Plasmid

#182762

Purpose

Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

Depositor

Qing-Jun Meng

Article

Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

Insert

mouse arntl promoter (Bmal1 Mouse)

Use

Lentiviral

Promoter

649 bp

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-NLRP3(R260W)

Plasmid

#73956

Purpose

Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

Depositor

Christian Stehlik

Article

Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

c-myc

Expression

Mammalian

Mutation

R260W

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
psPAX2-D64V-NC-MS2

Plasmid

#122944

Purpose

MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

NC-MCP (MS2 coat protein) fusion protein in Gag

Use

Lentiviral

Expression

Mammalian

Mutation

D64V

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.LSL.EGFP

Plasmid

#100047

Purpose

AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Hsp90 HA

Plasmid

#22487

Depositor

William Sessa

Article

Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

Insert

Hsp90 HA (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Mutation

Codon Optimised wtCNa

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUX4-WT

Plasmid

#99282

Purpose

All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1

Plasmid

#218796

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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