164,629 results

1. HA-GR

   Plasmid

   #215094

   Purpose

   Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   NR3C1 (NR3C1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-HcKCR1-EYFP

   Plasmid

   #182021

   Purpose

   Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

   Depositor

   Mingshan Xue

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR1
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Paxillin-22

   Plasmid

   #55114

   Purpose

   Localization: Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

   Insert

   Paxillin
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

   Plasmid

   #223712

   Purpose

   Expresses autophagy flux LC3B fluorescence reporter

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   LC3B (MAP1LC3B Human, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry::EGFP::LC3B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LBR pEGFP-N2 (646)

   Plasmid

   #61996

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   LBR (LBR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mARGAna (cassette 1)

   Plasmid

   #191341

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-PtenWT-Puro

   Plasmid

   #135676

   Purpose

   Constitutive expression (cMV promoter) of wildtype Pten cDNA, Puromycin selection

   Depositor

   Florian Karreth

   Insert

   Pten (Pten Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-NG-YE1-BE4max

   Plasmid

   #198535

   Purpose

   Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRFP-Lact-C2

   Plasmid

   #74061

   Purpose

   Fluorescent biosensor for phosphatidylserine.

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 (MFGE8 Bovine)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

    Plasmid

    #61629

    Purpose

    R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    mDII
    ntdTomato
    DII
    n3xVenus
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-synapsin-WHaloCaMP1a-EGFP

    Plasmid

    #205307

    Purpose

    Production of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK-ATF4

    Plasmid

    #26114

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Watermelon backbone

    Plasmid

    #155258

    Purpose

    Can be used to generate a Watermelon library

    Depositor

    Joan Brugge, Aviv Regev

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD/HisB-iRFP670

    Plasmid

    #45453

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    araBAD

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEmerald-CD36-C-10

    Plasmid

    #54030

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CD36 (CD36 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pCM184-ParB2::eGFP

    Plasmid

    #87252

    Purpose

    Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB2
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. TFORF0723

    Plasmid

    #142226

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EF-hGPC3

    Plasmid

    #24670

    Depositor

    Jorge Filmus

    Article

    Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

    Insert

    GPC3 (GPC3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pAAV-TRE-DIO-eGFP-2A-TetanusToxin

    Plasmid

    #166603

    Purpose

    Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

    Plasmid

    #208377

    Purpose

    Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human CMV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-MTS-jAspSnFR3-mRuby3

    Plasmid

    #203492

    Purpose

    Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    MTS-jAspSnFR3-mRuby3
    

    Use

    Lentiviral

    Tags

    His-tag, Mitochondrial localization signal, and m…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-Nucleolin

    Plasmid

    #28176

    Depositor

    Michael Kastan

    Article

    Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FLAG-tag

    Plasmid

    #55180

    Purpose

    FLAG-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    FLAG
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EF1a-mito-dsRED2

    Plasmid

    #174541

    Purpose

    mitochondria targeted dsRED expressed off of EF1a promoter

    Depositor

    Thomas Schwarz

    Insert

    Mito
    

    Use

    Lentiviral

    Tags

    dsRED

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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