We narrowed to 10,115 results for: HA

1. IC65 pEGFPN1 anti-IRSp53 nanobody

   Plasmid

   #135227

   Purpose

   Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171380

   Purpose

   Expression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized RfxCas13d
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HIV7-Bcl2-CD28-CD3z-T2A-EGFR

   Plasmid

   #163465

   Purpose

   Lentiviral vector encoding a Bim-specific chimeric antigen receptor with a synthetic Bcl2 binding element

   Depositor

   David Baker

   Article

   Lajoie et al Science. 2020 Sep 25;369(6511):1637-1643

   Insert

   Bim-specific CAR
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28b(+)_rPD-L1nb

   Plasmid

   #226715

   Purpose

   Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Use

   Tags

   HA tag and Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

   Plasmid

   #101064

   Purpose

   AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2_L398T
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-FLAG-AKAP-Lbc

   Plasmid

   #67571

   Purpose

   Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

   Depositor

   Graeme Carnegie

   Article

   Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

   Insert

   A-kinase anchoring protein Lbc (AKAP13 Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   R2604G in AKAP

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

7. GABA(A) receptor subunit a2SE

   Plasmid

   #49169

   Purpose

   pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

   Depositor

   Tija Jacob, Stephen Moss

   Article

   Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

   Insert

   GABA(A) receptor subunit alpha-2 (GABRA2 Human)
   

   Use

   Tags

   HA, bovine alpha 1 signal sequence, and pHGFP (pH…

   Expression

   Mammalian

   Mutation

   T343A

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P-rTetO-EGFP-T2A-RPL22-3xHA

   Plasmid

   #170326

   Purpose

   Tetracycline-inducible expression of HA and EGFP tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

   Plasmid

   #170327

   Purpose

   Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pan-Neurofascin (extracellular) scFv [A12/18]

    Plasmid

    #190485

    Purpose

    Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

    Plasmid

    #170317

    Purpose

    Expresses HA and EGFP tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pInducer-YAP1-S127A

    Plasmid

    #213584

    Purpose

    Doxycycline inducible expression of YAP1-S127A cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPB-cT3G-cERP2-MYC

    Plasmid

    #192899

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of MYC

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MigR1-AE9a

    Plasmid

    #12433

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Nat Med. 2006 Aug . 12(8):945-9.

    Insert

    AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AML-ETO protein with ETO exon 9a.

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AC50 pEGFPN1 anti-AMIGO-1 nanobody

    Plasmid

    #135225

    Purpose

    Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

    Plasmid

    #170320

    Purpose

    Expresses HA and mCherry tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

    Plasmid

    #224497

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pInducer-YAP1

    Plasmid

    #213583

    Purpose

    Doxycycline inducible expression of YAP1 wild type cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VGlut1 scFv [N28/9]

    Plasmid

    #190545

    Purpose

    Mammalian Expression of VGlut1 scFV. Derived from hybridoma N28/9.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    VGlut1 (Rattus norvegicus) recombinant scFV (Slc17a7 Mouse)
    

    Use

    Tags

    HA, 6xHis, Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

    Plasmid

    #78607

    Purpose

    A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short; U6

    Available since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

21. pPB-cT3G-cERP2-NR4A1

    Plasmid

    #192925

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR4A1 (NR4A1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB-cT3G-cERP2-WT1

    Plasmid

    #192906

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of WT1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

    Plasmid

    #170321

    Purpose

    Expresses HA and mCherry tagged RPL22, neomycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPB-cT3G-cERP2-NR1H2

    Plasmid

    #192919

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR1H2 (NR1H2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pInducer-YAP1-S127A/S397A

    Plasmid

    #213585

    Purpose

    Doxycycline inducible expression of YAP1-S127A/S397A cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S127A, S397A

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKH3-TRIM28-C65A/C68A

    Plasmid

    #92199

    Purpose

    Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    Tif1b (TRIM28 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Cysteines 65 and 68 to Alanines.

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #101061

    Purpose

    AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2-F391W
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA5-TEX264-deltaLRR-cSSH

    Plasmid

    #220210

    Purpose

    Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    c.4-75del / p.2-24del

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-nEF-dCas9

    Plasmid

    #106430

    Purpose

    Expresses SpdCas9 from the nEF promoter in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    nEF-dCas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NC14 pGLUE FBXW11

    Plasmid

    #36969

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    FBXW11 (FBXW11 Human)
    

    Use

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pPB-cT3G-cERP2-PPARG

    Plasmid

    #192912

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    PPARG (PPARG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPB-cT3G-cERP2-ZBTB16

    Plasmid

    #192903

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ZBTB16 (ZBTB16 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SARS_COV2_ORF10_HA_BASU

    Plasmid

    #153980

    Purpose

    Express Orf10 gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid

    #204215

    Purpose

    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    Cas13bt3 and U6.SapI sgRNA cloning site
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimisation by GenScript tool

    Promoter

    CMV/U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-ALK3 Q233D

    Plasmid

    #80874

    Purpose

    mammalian expression of ALK3 Q233D

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK3 (BMPR1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q233D (constitutively active)

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pPB-cT3G-cERP2-ATF4

    Plasmid

    #192911

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GST scFv [N100/13]

    Plasmid

    #190521

    Purpose

    Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GST (Schistosoma japonicum) recombinant scFV
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GC52 pEGFPN1 anti-Gephyrin nanobody

    Plasmid

    #135224

    Purpose

    Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHRIG-Akt3

    Plasmid

    #53596

    Purpose

    lentiviral expression of constitutively active human Akt3 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt3 (AKT3 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid

    #129025

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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