We narrowed to 22,191 results for: his

1. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTriEx4-Cdc42-2G

   Plasmid

   #68814

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-HP1beta

   Plasmid

   #17651

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 beta (CBX1 Human)
   

   Use

   Tags

   GFP and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-mE1

   Plasmid

   #32534

   Depositor

   Jorge Eduardo Azevedo

   Article

   Carvalho et al Mol Biotechnol. 2011 Oct 20.

   Insert

   E1, ubiquitin-activating enzyme (Uba1 Mouse)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBAD-Lime

   Plasmid

   #191255

   Purpose

   Bacterial expression plasmids for pH sensitive fluorescent protein Lime

   Depositor

   Robert Campbell

   Article

   Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

   Insert

   Lime
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a T7pCONS TIR-1 sfGFP

   Plasmid

   #154463

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMS31: pTwinStrep-SUMO-PmcTnsAB

   Plasmid

   #168164

   Purpose

   PmcTnsAB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsAB
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHM6-Q74

   Plasmid

   #40264

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSP1E2

   Plasmid

   #20063

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1E2 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1; contains repeats of he…

   Promoter

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUCBB-eGFP

    Plasmid

    #32548

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSP1E1

    Plasmid

    #20062

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeat of hel…

    Promoter

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET16b-Trx

    Plasmid

    #36944

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Human pIgR(SC)

    Plasmid

    #158216

    Purpose

    Express the ectdomain (SC) of human pIgR

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    PIGR (PIGR Human)
    

    Use

    Tags

    8×his

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMH-HA/Joseph2-Multitagged Plasmid

    Plasmid

    #226726

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pMH-HA/Joseph2
    

    Use

    Tags

    HA, Myc, GFP, Flag, V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET20b-delta133HtrA2

    Plasmid

    #14126

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAcGP67A-PSMA

    Plasmid

    #11524

    Depositor

    Pamela Bjorkman

    Article

    Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

    Insert

    prostate-specific membrane antigen (FOLH1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    There is a short N-terminal truncation of this pr…

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTcI39-switch

    Plasmid

    #195557

    Purpose

    Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mRFP1
    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAD/CMV/V5-DEST-Cdc42-2G

    Plasmid

    #68812

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Adenoviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 CYP2D6

    Plasmid

    #67090

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2D6 (CYP2D6 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAPtag5-IgK-NCAN-Full length

    Plasmid

    #219812

    Purpose

    Expresses His-tagged NCAN-Full length to produce recombinant protein

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    IgK (Igk Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEFmEGFP-mDia2

    Plasmid

    #25407

    Depositor

    Arthur Alberts

    Article

    Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

    Insert

    mDia2 (Diaph3 Mouse)
    

    Use

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Encodes mDia2

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSP1E2D1

    Plasmid

    #161606

    Purpose

    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E2D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NbV5-Rluc8

    Plasmid

    #201480

    Purpose

    BRET detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    Rluc8-NbV5
    

    Use

    Tags

    Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GreenT-EC - pRSET

    Plasmid

    #193947

    Purpose

    Bacterial expression vector for a low-affinity calcium indicator

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST26-USP47

    Plasmid

    #64326

    Purpose

    Mammalian expression and purification of USP47.

    Depositor

    Grigory Dianov

    Article

    Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

    Insert

    USP47 (USP47 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB_TRE_sg30-PDGFR_EF1a_mCherry

    Plasmid

    #186309

    Purpose

    Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg30-PDGFR
    mCherry
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pProEx-IDE-wt

    Plasmid

    #99014

    Purpose

    Express human Insulin Degrading Enzyme (IDE) in E. coli.

    Depositor

    Wei-Jen Tang

    Article

    Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

    Insert

    insulin degrading enzyme (IDE Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    TRC

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3_2FLAG-AKAP79ΔCaM

    Plasmid

    #183453

    Purpose

    Expression of human AKAP79 lacking the calmodulin binding site (Δ79-86)

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    aa79-86 deleted

    Promoter

    CMV

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Use

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-28a+GFP-CAMSAP3 CKK

    Plasmid

    #59033

    Purpose

    expresses CKK domain of CAMSAP3 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CKK domain (CAMSAP3 Mouse)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

33. Integrin-β6 I domain I270C

    Plasmid

    #159639

    Purpose

    Contains integrin beta 6 residues 108–352 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

    Insert

    Integrin beta 6 I domain I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E1D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NbV5-HALOTAG

    Plasmid

    #201482

    Purpose

    Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-HALOTAG
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-15b PAI-RBP

    Plasmid

    #45390

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pET21c(+)-mtLeuRS

    Plasmid

    #31079

    Depositor

    Linda Spremulli

    Article

    Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

    Insert

    Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Nt …

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pETCK3 TGP-E

    Plasmid

    #236255

    Purpose

    Expresses TGP-E, a thermostable fluorescent protein

    Depositor

    Natasha DeVore

    Article

    Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

    Insert

    Thermal Green Protein Q66E (TGP-E)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac operon

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBAD-tdTEVDB_C-15A/G4C

    Plasmid

    #177205

    Purpose

    Permuted td intron with evolved initiation sequence for the expression of circular mRNA

    Depositor

    Stephen Fried

    Article

    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

    Insert

    Permuted GFP with evolved initiation sequence for circular mRNA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Permuted sfGFP after residue 52 & Changed Cyt…

    Promoter

    araBAD

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Integrin-αv Headpiece M400GC

    Plasmid

    #159637

    Purpose

    Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    alpha v headpiece M400GC
    

    Use

    Tags

    6x His and Strep II

    Expression

    Mammalian

    Mutation

    M400C

    Promoter

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge