We narrowed to 12,771 results for: NUC

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pYPQ141-ZmUbi-RZ-Fn

Plasmid

#108864

Purpose

FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Type

Empty backbone

Use

CRISPR; Gateway compatible crrna entry clone

Expression

Plant

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSC5GGv2

Plasmid

#188604

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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LacI-11/109-pHG165c

Plasmid

#90059

Purpose

Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI-11/109

Expression

Bacterial

Mutation

109

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_IRES-blast

Plasmid

#166411

Purpose

express hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Promoter

ef-1a

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Purpose

Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin, CMV

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
EGFP-Ki-67(RASA)-mCherry_IRESpuro2

Plasmid

#182921

Purpose

Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

MKI67 (MKI67 Human)

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Mutation

RASA mutation

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-YAP-deltaC

Plasmid

#21126

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

EGFP-C3

Expression

Bacterial and Mammalian

Mutation

5 Carboxy-terminal amino acids -FLTWL are deleted

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

Plasmid

#42046

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

F951A, F955A, I958A, F960A

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDR348 FANCF

Plasmid

#47510

Purpose

human gRNA expression vector targeting FANCF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-FANCF (FANCF Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pUDE1093

Plasmid

#204227

Purpose

Episomal yeast plasmid expressing the Ercas12a

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Insert

Eubacterium rectale cas12a (Ercas12a)

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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LLP784_SnoopCatcher-DNMT3A-CO

Plasmid

#211777

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Spy-EZH2

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut

Plasmid

#42045

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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HS-EB3-GFP

Plasmid

#105948

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLin28A-myc-T2A-tagRFP

Plasmid

#140267

Purpose

Expresses LIN28A in mammalian cells

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

LIN28A (LIN28A Human)

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pC012 LwCas13a locus with nontargeting spacer

Plasmid

#91904

Purpose

LwCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with non-targeting spacer

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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VVT1

Plasmid

#65779

Purpose

The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SaCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp30-C1

Plasmid

#134547

Purpose

Expresses YFP-Rpp30 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp30 (RPP30 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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LLP787_SnoopCatcher-EZH2-FL-CO

Plasmid

#211780

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP780_SpyCatcher-KRAB-CO

Plasmid

#211773

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-KRAB

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNXF-1-5xmut_O

Plasmid

#147116

Purpose

Mammalian Expression of HsNxf-1-5xmut

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HsNxf-1-5xmut (NXF1 Human)

Expression

Mammalian

Mutation

See Depositor Comments Below

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pAM4951 (pCV0063)

Plasmid

#120084

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pIRESneo-Ago2

Plasmid

#215841

Purpose

Expression of AGO2

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human AGO2 (AGO2 Human)

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pWPT-mCherry

Plasmid

#190605

Purpose

Obtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Mutation

EGFP Kozak sequence has been replaced with a stre…

Promoter

EIF1-short

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAGE2.0-i

Plasmid

#194639

Purpose

Empty pAGE2.0 plasmid with pBAD inducible promoter

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p45)

Plasmid

#135942

Purpose

Expresses AUF1p45 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJT25_GalL_BE3

Plasmid

#143736

Purpose

Expresses BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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