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We narrowed to 48,680 results for: TOR

Showing: 7021 - 7050 of 48680 results
  1. pDONR P2R-P3-SV40 polyA signal

    Plasmid
    #48627
    Purpose
    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.
    Insert
    SV40 early polyadenylation signal cassette
    Use
    Gateway Cloning
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available Since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available Since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Expression
    Mammalian
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. MSCV-FlagmuEzh2deltaset-Hygro

    Plasmid
    #49403
    Purpose
    Expresses a Delta-set deletion mutant of Flag Tagged Ezh2. Hygromycin resistant.
    Depositor
    Insert
    3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain
    Use
    Retroviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Set domain deleted
    Promoter
    MSCV promotor
    Available Since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 7021 - 7050 of 48680 results