We narrowed to 165,307 results for: addgene

1. pWN_U6-B2M-miniGag

   Plasmid

   #228957

   Purpose

   miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag
   

   Expression

   Mammalian

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TNFa-GFP

   Plasmid

   #163660

   Purpose

   Expresses GFP tagged TNFa in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   tumor necrosis factor (TNF Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9 sgRNA vector

   Plasmid

   #68463

   Purpose

   Cas9 sgRNA vector for cloning

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-cGAS-HA

   Plasmid

   #130910

   Purpose

   Expresses human cGAS-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGAS
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMBEC6

   Plasmid

   #187598

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMV-eGFP

   Plasmid

   #193022

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-KRAB-dCas9-IRES-BFP

   Plasmid

   #85449

   Purpose

   Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. BRCA2 A11.2 gRNA

   Plasmid

   #90550

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A11.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCas9-T2A-BFP

   Plasmid

   #78547

   Purpose

   Cas9 protein with BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF-XMAS-1xStop

    Plasmid

    #164411

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Parkin

    Plasmid

    #213547

    Purpose

    Transient mammalian expresssion of untagged Parkin

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-PIG-Cre

    Plasmid

    #50935

    Purpose

    retroviral expression of Cre recombinase

    Depositor

    Pier Pandolfi

    Article

    Chen et al Nature. 2005 Aug 4;436(7051):725-30.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158972

    Purpose

    Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-kdrl:mCherry-caax

    Plasmid

    #194287

    Purpose

    Expression plasmid for zebrafish or Danionella cerebrum

    Depositor

    Pui-Ying Lam

    Article

    Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

    Insert

    kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
    

    Use

    Zebrafish or danionella cerebrum expression

    Tags

    mCherryCAAX from the Tol2kit (Kwan et al., 2007)

    Promoter

    kdrl(flk1)

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMGS7 (AAVS1sgRNA)

    Plasmid

    #126582

    Purpose

    A CRISPR/Cas9 construct to target the AAVS1 locus in humans

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AAVS1 sgRNA
    

    Expression

    Mammalian

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDT-SuperFi-Cas9

    Plasmid

    #183630

    Purpose

    Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

    Depositor

    David Taylor

    Article

    Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

    Insert

    SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

    Promoter

    T7

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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