We narrowed to 29,515 results for: REP

1. pENTR221-IL6.Pro

   Plasmid

   #79491

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Il6 Promoter (IL6 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   IL6 Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE-HA-Ku70-siR-WT

   Plasmid

   #82329

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

   Insert

   Ku70 (XRCC6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-tet

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcmv3-IRG1-L272Q-HA

   Plasmid

   #198184

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The leucine in IRG1 at position 272 mutates to gl…

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4/TO/GFP-ZNF598

   Plasmid

   #141191

   Purpose

   Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   ZNF598 (ZNF598 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mChF-AIP

   Plasmid

   #61527

   Purpose

   Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   FK506 binding protein 1A (FKBP1A Human)
   

   Use

   Tags

   AIP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLV-CMV-XRCC1-EGFP-Hygro

   Plasmid

   #176062

   Purpose

   EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   X-ray repair cross complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL8 lenti-D10Acas9-3xflag-blast

   Plasmid

   #112134

   Purpose

   lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   D10Acas9-3xflag-blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A mutant in Cas9

   Promoter

   EF1a

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLM-AMA18.0 dCas9-VPR

   Plasmid

   #138945

   Purpose

   Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

   Insert

   p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
   

   Use

   CRISPR and Synthetic Biology; Ama1 autonomously r…

   Tags

   NLS

   Expression

   Mutation

   Promoter

   p40S (AN0465) Aspergillus nidulans

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFPV25-cR-2iG

   Plasmid

   #204618

   Purpose

   Fluorescent reporter for SPI2 induction in Salmonella

   Depositor

   Jacques Neefjes

   Article

   Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

   Inserts

   rpsM promoter
   sseA promoter
   

   Use

   Tags

   GFP and dsRed

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

    Plasmid

    #62953

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLX_TRC311 ETV1

    Plasmid

    #74981

    Purpose

    Constitutive ETV1 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid

    #122056

    Purpose

    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P

    Depositor

    Stephen Tapscott

    Article

    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

    Insert

    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    MyoD chimeric protein substituted with the NeuroD…

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #105000

    Purpose

    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

    Plasmid

    #62952

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVC-DNAJB6b

    Plasmid

    #175065

    Purpose

    expresses mVenus and mCherry tagged DNAJB6b in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    DNAJB6b (DNAJB6 Human)
    

    Use

    Tags

    V5 and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PXPR007 sgCIC-2

    Plasmid

    #74959

    Purpose

    Cas9 + sgCIC-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA4TO-3XFLAG Halo WT nsp1

    Plasmid

    #175412

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1 (ORF1ab CoV2)
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-COSMC-KO

    Plasmid

    #80008

    Purpose

    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    COSMC (C1GALT1C1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDipA-TEV-His12

    Plasmid

    #137042

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG426GAL-EGFP

    Plasmid

    #166143

    Purpose

    EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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