We narrowed to 11,999 results for: SHA

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pFUW-tetO-ID2

Plasmid

#139808

Purpose

doxycycline-inducible expression of Human ID2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

ID2 (ID2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TOMM20

Plasmid

#207789

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L858R/T790M)

Plasmid

#32073

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L858R/T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-GOLGA2

Plasmid

#207791

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1a-FB-dCas9-puro

Plasmid

#100547

Purpose

Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Inserts

N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin

Use

CRISPR

Tags

FLAG, Biotin-acceptor-site (Avitag)

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RAD21 C-tag CRISPR

Plasmid

#140540

Purpose

RAD21 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-WPRE

Plasmid

#130994

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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p23-mNeongreen-DDX21 WT-mRuby3

Plasmid

#179225

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Tags

mNeongreen and mRuby3

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-J chain

Plasmid

#145146

Purpose

Mammalian expression plasmid for J chain of polymeric antibody classes

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

immunoglobulin J chain (JCHAIN Human)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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Arf4-GFP

Plasmid

#39556

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylaion factor 4 (ARF4 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX-MCS-ASC-GFP

Plasmid

#73957

Purpose

Express human ASC with a C-terminal EGFP tag in mammalian cells

Depositor

Christian Stehlik

Article

de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2

Plasmid

#40260

Purpose

RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Insert

mRuby2

Tags

His6 and Xpress

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130999

Purpose

Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

Ef1a

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

Plasmid

#130998

Purpose

Double floxed ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

Ef1a

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-LAMP1

Plasmid

#207787

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Clover

Plasmid

#40259

Purpose

GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Insert

Clover GFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pFB2B

Plasmid

#195284

Purpose

Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Puro

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

Plasmid

#231986

Purpose

Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

OVA-2A-ZsGreen

Use

Lentiviral

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Meis1

Plasmid

#139824

Purpose

doxycycline-inducible expression of mouse Meis in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Meis1 (Meis1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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SKIV2L

Plasmid

#156014

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SKIV2L (SKIC2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

D3E

Promoter

EF-1a

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM16F-M522K-peGFP-N1

Plasmid

#228579

Purpose

Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

M522K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT

Plasmid

#238281

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Cebpa

Plasmid

#139834

Purpose

doxycycline-inducible expression of mouse Cebpa in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Cebpa (Cebpa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2 GFP-Rtn4a

Plasmid

#86684

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-ICUE3

Plasmid

#61623

Purpose

Plasma membrane-targeted FRET biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

Lyn-ICUE3 (RAPGEF3 Human)

Tags

ECFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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TLCV2-Scal-T2A-EGFP-Puro

Plasmid

#234949

Purpose

Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI-T2A-EGFP-Puro

Plasmid

#234948

Purpose

Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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